Microbial Community In Core Collected From DK-2Gas Hydrate Research Well, Qilian Moutains

In2009, since gas hydrate had been obtained in Qilian Mountainpermafrost, Qinghai Province, China became one of countries possessing richgas hydrate. As a new kind of energy, gas hydrate is important for energypolicies. In the ocean where gas hydrate has been research thoroughly,microbes involves in the process of methane metabolism, which is importantfor maintaining balance of global methane. Comparing with in the ocean, gashydrate in the subsurface is easier for exploration and development. Sodeveloping microbiology research on gas hydrate in Qilian Mountainpermafrost is of significant value.11cores, from DK-2gas hydrate researchwell in Qilian Mountain permafrost, was gathered in147.80~282.10m insubsurface as research objects. The research on the relationship ofmicroorganism and gas hydrate, consist of microbial activity in the process ofmethane formation, microbial activity in the process of methane consumption,and microbial community characteristics in gas hydrate, to describe microbial characteristics in gas hydrate riching area in Qilian Mountain permafrost. Inour research, we found that:1. There was no methanogen in detecting bacterial and archaeal16SrDNA, resulting in microbial activity is less in the process of methaneformation.2. There was no aerobic or anaerobic methane oxidation typical processin detecting gene of aerobic methane oxidation process(pmoA, mmoX andmxaF), gene of ANME (mcrA) and bacterial16S rDNA, indicating thatmethane consumption in gas hydrate had been obtained in Qilian Mountainpermafrost are particular.3.8groups of bacteria (Actinobacteria, Firmicutes,-Proteobacteria,-Proteobacteria,-Proteobacteria,-Proteobacteria, Bactemides andDeinococcus-Thermus) were found in detecting bacterial16S rDNA, thenumber of the strains of which reach157, indicating that the bacteria detectedin our research corresponds with characteristics of the environment inpermafrost, and the microbial species decreased as the depth increasing.4. Comparing gas hydrate samples and non-gas hydrate samples, we cansee that:(a) The number of-Proteobacteria clone is bigger in non-gashydrate samples than in gas hydrate samples, and Stenotrophomonas andNevskia were only found in gas hydrate samples but Moraxella, Shigella andProteus were often found in non-gas hydrate sample.(b) Arthrobacter couldbe found in all non-gas hydrate samples, but only found in one gas hydrate sample DK2-25.