The Qilian Permafrost Zone Of Gas Hydrate Culture Microbial Analysis And Identification Of A New Species Of Bacteria

Gas hydrate is a new green energy, mainly distributed in thedeep-sea sediments and terrestrial permafrost. According to statistics, theglobal gas hydrate resource is about2.1×1015m2. Because of the hugepotential for energy and environmental effects of gas hydrate, in themid-20th century, especially in the lower lobe, the world set off the crazeof gas hydrates. In September2008, our exploration of Jurists home inQinghai Qilian Mountain permafrost zone drilled gas hydrate samples ofthis great discovery of our country to become the first drilled gas hydratein the low-latitude countries, and China has become fourth hydrate foundin the permafrost zone countries. Ago, so far, the global gas hydrate stillshallow gas hydrate research in China started late, a lot of technology tolag behind other countries, the countries have given a high priority. As theworld's third permafrost, and favorable terrain, China has the potential tofind more gas hydrate deposits. Qinghai plateau permafrost zone of gashydrate exploration success has attracted a high degree of attention athome and abroad, this finding is another major breakthrough in the energy field in China. So far, however, Geomicrobiology of gas hydrate ismainly concentrated in the deep sea units within the gas hydrate-richdeposit area results in microbiological research, little progress in thepermafrost zone of gas hydrate research, only about Canada Mackenzie inthe mining and American and Alaska mountains permafrost hydrateresearch, and research of microbial small, domestic for the study of thisone also in a blank state. According to the seabed gas hydrates andmicrobes to combine aspects of the research base, to analyze thepermafrost gas hydrate in microbiological research and addicted toisolated and cultured (a) cold-microbial resistance analysis, simulation ofmethane-oxidizing bacteria culture, as well as other analysis of relatedstrains, and explore its distribution law, the purpose is to establish acomprehensive MPOG technology. The existence of a wide range of oilshale in the Qilian Mountain permafrost zone, so this topic under thebacking of the Qilian Mountain permafrost zone of gas hydrate microbialresearch, the microbes in the oil shale region also made a preliminaryseparation was isolated with a view to the microbes with the trend of oilpotential. Detailed analysis in the core samples collected since the fewstudies of the Qinghai-Tibet Plateau, so the separation filter out more of anew species of microbes, this article only a new species of bacteria,microbes were isolated and cultured. The main content of this researchproject: 1. Culturable microorganisms on the Qinghai Qilian Mountainpermafrost gas hydrate core samples in kind. Four kinds of medium buildcapacity of permafrost natural gas hydrate bacteria in the cultureconditions of different temperatures the same pH value, compare, filterout the best training methods, and on the conditions of the mediumPYGV appropriate adjustments. On in PYGV medium, after24weeks ofculture observed statistical DK2drilling cores were isolated from4738colonies were isolated and identified95bacterial, belonging to12genera.DK-6core samples3442single colony count of110bacteria wereisolated and identified, belonging to27genera. Can nurture the fourbacterial microbial separation process. Bacilli door after the bacteria16SrDNA strain identification results showed that the culturablemicroorganisms sequence DK2and DK6-drilling cores isolated in thephylogenetic tree clustering for the two main systems taxa:Gram-negative flora deformation and Gram-positive flora, which Proteusdoor (Proteobacteria,), the distribution of alpha-, beta-, of γ-andδ-deformation in Bacillus sub-group; Gram-positive flora contains high(G+C) mol%of the actinomycetes door (Actinobacteria), low (G+C)mol%of Firmicutes (Firmicutes) and of CFB flora the the bacillus door(Bactemides), and abnormal cocci-Thermus bacterial phyla(Deinococcus-from Thermus). Found through the analysis of the coreburning loss of environmental factors and microbial species, the distribution of microorganisms affected by the environment is very large,have a close relationship with the underlying construct has a symbioticrelationship between micro-organisms. Microorganisms and coregeological environment together constitute the Qilian Mountainpermafrost zone of gas hydrate stability system.2. Microbial separation of oil shale core permafrost zone in Qilianscreening and microbiology laboratories, culture has been preservedstrains (Karamay Oilfield fermented seeds), a total of eight thechemotactic oil capacity high-purity microbial. This eight microbial oilacclimation, which two can only grow in the oil medium, and the othersix significant oil accumulation effect. These eight microorganisms were16S rDNA strain identification and to determine its classification andphylogenetic relationships of strains.3. New species identification of new species of bacteria found in theQilian Mountain permafrost zone of gas hydrate culturablemicroorganisms separation process and determination of the16S rDNAsequences of new species of microbes, such micro-organisms belongingto Hymenobacter the case, with the development of the most similarstrains of H. desert,96%similarity. Experiments further confirmed thatthe medium and the optimum culture conditions of new species ofmicrobes, bacteria, Gram-negative bacteria, cytochrome oxidase positive,catalase-positive. Measured strains of physiological and biochemical indices, and cellular components. Determine new species of the genus ofthis species Hymenobacter.