Research On Preparation And Properties Of Composite Electrospun Tissue Engineering Scaffolds

Electrospinning technology (electrospinning for short) is considered to be an effective method in preparation of tissue engineering scaffolds. But there still is a relatively large optimization space. In this paper, tissue engineering scaffolds suitable for cell growth with releasing function have been studied from the following three aspects:1) Studied in the properties of PLGA [poly(lactide-co-glycolid)] electrospun fibers of different molecular weights or ratio, and adjusted the aperture of fiber scaffold by changing receiving electrode. The results of study showed that different PLGA fibers displayed big differences in their properties. The PLGA grid-type fiber membrane was prepared using copper grid as a receiving electrode. Its aperture was larger and it was more conducive for cells growting inside than the PLGA non-woven fiber membrane.2) Changed biocompatibility of fiber scaffold by using coaxial electrospinning technology and compounding materials. PLGA/St (starch) fiber was successfully prepared by coaxial electrospinning technology. Starch adding to the fiber had effectively improved hydrophilicity and degradation and mechanical properties of PLGA fiber. Cell culture showed that PLGA/St fiber membrane had good biocompatibility. Meanwhile, CS (chitosan)/PLLA (poly-l-lactic acid) fiber was successfully prepared. A small amount of chitosan component in the fiber could not improve hydrophilicity, and reduce the mechanical properties, but effectively improved water-absorption and degradation of PLLA fibers. The cells had higher adhesion rate and activity on the CS/PLLA fiber membrane than the PLLA fiber membrane.3) By establishing core /shell structures, controllable releasing systems of scaffold were built. Using BSA (bovine serum albumin) as the model protein, the core /shell PEO (polyethylene oxide)/PLGA fiber loaded with BSA was prepared. The fiber had a clear core /shell structure and good releasing property of BSA. The influence of voltage, flow and pole distance on coaxial electrospray was also studied in the paper. PLGA microsphere and PLGA microsphere packaging BSA were prepared. And directly spray method could be a feasible method for the combination between microspheres and fiber scaffolds.