Study On Influential Factor To Methane Metabolic Of Methane-utilizing Bacteria And Methoanol Detection Method

The conception, classification and metabolic pathway of methane-oxidizing bacteria were accounted. The category, construction, regulation and mechanism of MMO were introduced, too. The forword study of methane-oxidizing bacteria and MMO, which included optimization of culture condition, synthesis of chemical substances, genetic engineering, monitoring and administering of methane, were also described. Moreover, the application of methane-oxidizing bacteria and MMO in environmental pollution control, methane drainage control and coal methane control etc were summarized. Finally, the contents and significance of this paper were presented.Experimental installation was constructed, and the influential factors (contained temperature, Nitrogen, CO2) to its detection stability were studied. The results displayed that the experimental installation owned perfect detection stability for experiment of study on influential factors to methane metabolic by ME16.Aforementioned experimental installation was used to study the influential factors to methane metabolic by Pseudomonas aeruginosa ME 16. Results showed that the optimum temperature was 30℃, the optimum pH was 7 and the optimum NaCl concentration was 0.5%. When the proportion of methane and oxygen concentration was 1, the ability of methane metabolic by ME 16 could get the optimum level. The Pb2+and Cu2+owned inhibition effect to methane metabolic by ME 16. As EDTA concentration was greater than 15 mmol/L, the ability of methane metabolic by ME 16 was observably restrained. Moreover, if the rest cell PBS contained 10 mmol/L EDTA, the inhibiting effect of Cu2+and Pb2+to the ability of methane metabolic by ME 16 was avoided. Methanol at high level could inhibit the ability of methane metabolic by ME 16, but at low level, could improve the ability of methane metabolic by ME 16.Methylobacterium organophilium ME25 was used to construct detection system to detect methanol. The result showed that detection system had larger linear range from 0.0% to 2%, and the detection limit for methanol was 3.4×10-4%.The response time of the detection system was around 30 min. The detection system had relatively perfect specificity to methanol and almost perfect storage stability and reproducibility.