Study On Allelopathy Of Root Exudates Of Three Species Michelia

In this study, Allelopathy of the root exudates of Michelia maudiae, Michelia platypetala and Michelia macchurel which were extracted with aquiculture, aqueous extract of soil and aqueous extract of root were studied. Allelopathic Effects and mechanism of Robinia pseudoacacia, Raphanus sativus, Lolium multiflorum were evaluated. Phenolics were separated successfully with high performance liquid chromatography (HPLC). The result showed as follows:1. Root exudates of 3 species Michelia maudiae by Aquiculture and aqueous extract of root reduced the germination and growth. Root exudates of Michelia maudiae and Michelia platypetala by aqueous extract of soil had slight auxo-action or inhibiting effect.2. Root exudates of 3 species Michelia maudiae indicated soluble protein,but enhanced MDA content. Root exudates of Michelia maudiae and Michelia platypetala by aqueous extract of soil slightly promoted SOD activity and POD activity of Raphanus sativus. Root exudates of Michelia maudiae, Michelia platypetala by aquiculture and aqueous extract of soil made the SOD activity and POD activity increased in varying degree. Other treats all decreased the SOD activity and POD activity.3.1,4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, benzoic acid and cinamonic acid were found in all the root exudates of Michelia maudiae, Michelia platypetala by aquiculture and aqueous extract of soil, and the content of phenolics were different. The root exudates of Michelia maudiae, Michelia platypetala by aqueous extract of roots were no benzoic acid. In total Phenolics contents, the root exudates by aquiculture had the most Phenolics, and the root exudates by aqueous extract of roots had more than the root exudates by aqueous extract of soil. 1,4-hydroxybenzoic acid and ferulic acid were significantly higher than other in the root exudates.4. Total Phenolic contents exudates of root of 3 species Michelia maudiae was positively correlated with synthetic allelopathic effects of three tested plants. The content of benzoic acid had negative correlation with the allelopathic effects of Raphanus sativus tested by Michelia maudiae, Michelia platypetala and Michelia macchurel. There was positive correlation between the content of the rest 4 species phenolics and the allelopathic effects of Robinia pseudoacacia, Raphanus sativus, Lolium multiflorum tested by Michelia maudiae, Michelia platypetala and Michelia macchurel. Therefore, allelochemicals of Phenolic of Michelia maudiae, Michelia platypetala and Michelia macchurel had various allelopathy to Robinia pseudoacacia, Raphanus sativus, Lolium multiflorum.