The Bioinformational Analysis In Brachypodium MADS-box Genes A Dissertation In Biophysics

Brachypodium distachyon, has been considered as a new potential model plant species for structure and genome research of Triticeae. In recent years, it has been studied extensively because of its small genome, less chromosomes, rapid life cycle, higher seed yield, and close relationship with Triticeae. MADS-box transcription factors, besides being involved in floral organ specification, have also been implicated in several aspects of plant growth and development.Thus, an analysis involving structure, expression and functional aspects of MADS-box genes in Brachypodium was required to understand the magnificant role of this gene family in reproductive development and molecule mechanism.The results as follow:1 Two new MADS-box genes are predicted through BLASTing the similar sequences.2 The analysis of motifs in 57 MADS-box genes shows that MADS domain is the indentity motif of the MADS family, K-box Domain is the indentity motif of the MICK subfamily, N-terminal, Coiled coil region and unknown motifs are not indentified in any subfamily.3 From the Phylogenetic analysis made by rice and Brachypodium MADS-box genes, we can see 37,7,6,4,3 genes belongs to the MIKCC,MIKC*,Mα,Mβand Mγsubfamily, respectively.At the same time,24 orthology genes were found.4 The analysis of chromosomal location of 57 Brachypodium MADS-box genes, we found 19,13,14,8 and 5 genes located in Chromosome 1-5. Through the selection pressure analysis, typeⅡgenes were more conservative than typeⅠgenes.5 The specific expression analysis of 6 genes which were predicted in the function of stress resistance and tissue-specific expression turns out the predict is absolutely right.The result is familiar with the report of Rice.