Investigation Of The Interaction Of DNA With Amphiphilic Substances And Metal Complexes

Nucleic acids contain the genetic instructions used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information. The research about the interaction of nucleic acids with other molecules is an important field in bioanalytical chemistry. In addition, nucleic acid is of great significance in the research and development of pharmaceutical products, biochemical reactions of nucleic acids, as well as in disease diagnosis and clinical medicine. Therefore, focusing on the development of high sensitivity and selectivity of rapid determination method of nucleic acids, further research for the discussion of interaction mechanism between nucleic acid and molecules have become the current hot topic in analytical chemistry.Based on the resonance light scattering (RLS), fluorescence and UV-Vis spectra techniques, we studied the interaction of amphiphilic substances and metal complex with nucleic acids, and established sensitive methods for quantitative determination of nucleic acids. Furthermore, interaction mechanism was studied and discussed with other multiple techniques. The contents of this thesis mainly including three parts, listed as follows.1. The interaction between fluorinated surfactant potassium perfluorobutane sulfonate (FC-98) and DNA was studied by resonance light scattering (RLS) spectra. From the research, we found that when DNA was added to FC-98, RLS intensity was enhanced obviously, the enhanced intensity and the concentration of DNA was linear in a certain range. Based on this, a quantitative determination method of DNA was established. Under optimum conditions, when the concentration of FC-98 was 4.0×10-6 mol/L, the linear range of DNA was the widest, for 0.62-6.08 mg/L, the detection limit (3δ) was 31.0 ng/mL. Combining with UV-Vis, IR spectra and TEM technique, we studied the interaction mechanism between FC-98 and DNA. The results showed that hydrophobic force played a major role in binding, besides, the interaction caused the changes of DNA configuration.2. The interaction between Cationic fluorinated amphiphilic polymer P(HFMA-St-MOTAC)-g-PEG and DNA was studied by resonance light scattering (RLS) spectra. It is found that RLS intensity was enhanced obviously when DNA was added to P(HFMA-St-MOTAC)-g-PEG, the enhanced RLS intensity was proportion to the concentration of DNA in the range of 0.92-6.08 mg/L, the detection limit (3δ) was 37.0 ng/mL, for the determination of synthetic samples with satisfactory results. With UV-Vis, IR...