The Herbal Compound Yi Tang Kang Apoptosis In Rat Islets

Purpose:1.Through this experiment, summarized in vitro isolation, cultivation methods in primary islet cells.2.Observation with Chinese Medicine"Yi Tang health"containing serum on cultured primary rat islet cell apoptosis is inhibited or reduce the apoptosis.Material and method:We chose the normal feeding Wistar rats were treated with a collagenase digestion to separate the islets, with 20 projects and 80 aim stainless steel mesh screen to filter the purified islets. To gain a higher activity of the islet, and then after overnight culture, the activity of insulin and then higher again after trypsin digestion. Make it into islet single cell suspension, and then Ham's F-10 medium (with 1% fetal bovine serum, 2mmol / L of L-glutamine) washed and transferred to 24 well plate. Selection medium DMEM medium containing glucose concentrations of 22.2 mmol ? L-1. Join the single cell suspension of each hole 250 slightly, adding serum interference was observed in serum of three time points after the intervention of islet cell apoptosis, namely the serum 8 hours after intervention, 16 hours and 24 hours. Then all the data for statistical analysis, the outcome.Results:1,Islet isolation and culture shape and structural integrity, higher activity.2,After digestion of the islets after incubation, a dispersed islet cells, can succeed by providing the corresponding experimental islet scattered single-cell volume.3,After statistics showed that intragastric Chinese Medicine "Yi Tang health" intervention group serum islet cell apoptosis, at the same time point, significantly lower than the serum in the intervention group fed distilled water.Conclusion: 1,Wistar rats were separated from the living islets obtained after, another after trypsin digestion, the formation of a single cell suspension, is a activity of islet cells, the serum can interfere with the next step; by this method can separate living islets, islet cells are primary.2,Isolated primary islet cells during serum after the intervention, measured at different time points of islet cell apoptosis, at the same time, clearly show that orally Chinese medicine"Yi Tang Kang", islet cells in serum interfere with withered death rate was significantly lower than serum levels of intervention administered distilled water islet cell apoptosis. Further proves that Chinese medicine"Yi Tang Kang", can inhibit or slow down the rat islet cell apoptosis.