Infected With Candida Albicans In Mice Kidney Index And Disease Correlation And Its Application In Traditional Chinese Medicine Research

ObjectiveTo explore the relationship between the weight index of kidneys and prognosis in the mice with Candida albicans infection. The renal index was used to evaluate the mice model with Candida albicans infection and the treatment efficacy with some related medicine. An convenient and objective Indicator system for evaluation of the model was established.Materials and methodsPart1:120 Kunming mice, weighing 20±2g, half male and half female, were randomly divided into four groups:Candida albicans of high-dose group (A group), Candida albicans of medium-dose group(B group), Candida albicans of low-dose group (C group), and control group (D group),each group was 30 mice. The mice of each group were raised adaptively a week, then except the control group, the mice of each group were injected the corresponding amount of Candida bacilli via the tail vein: The Candida bacilli concentration of A group was 4×106CFU/ml, B group was 2×106 CFU/ml, C group was 1×106 CFU/ml, the amount of Candida bacilli was 0.1ml/10g. The mice of each group were fed normally after infected Candida albicans.From the fifth day after infection, five mice were randomly selected from each group every other day (ie: From the first 5 days, 7 days, 9 days, 11 days, 13 days, 15 days), then the renal index, blood, Bacterial load in blood and kidney, kidney pathology (HE staining and PAS staining), body weight and so on were detected.Part2:120 Kunming mice, weighing 20±2g, half male and half female, were randomly divided into four groups:Candida albicans group (A group), Candida albicans plus allicin treatment group (B group) , Candida albicans plus fluconazole treatment group (C group), and control group (D group),each group was 30 mice. The mice of each group were raised adaptively a week, then except the control group, the mice of each group were injected Candida bacilli via the tail vein: The Candida bacilli concentration was 2×106 CFU/ml, the amount of Candida bacilli was 0.1ml/10g. The mice of each group were fed normally after infected Candida albicans, the next day after infection, the mice of B and C group were administrated drug respectively, once a day until the end of the experiment.From the fifth day after infection, five mice were randomly selected from each group every other day (ie: From the first 5 days, 7 days, 9 days, 11 days, 13 days, 15 days), then the renal index, blood, Bacterial load in blood and kidney, kidney pathology (HE staining and PAS staining), body weight and so on were detected.Results1. Compared with the control blank control group, the renal index of the mice of high-dose, medium-dose, low-dose Candida albicans infection group were significantly increased (p<0.01)2. The differences of bacterial load of kidney of each group were similar (p<0.01)3. Bacterial load of blood were not significant difference among all groups.Conclusion:1. Renal index of the mice with Candida albicans infection was positively correlated with the amount of bacterial load and the pathological changes in kidneys.2. Renal index can accurately reflect the body's level of Candida albicans infection. It is more operable to consider renal index as the evaluation criteria of deep Candida albicans infection disease models. Compared with other indicators, renal index is more convenient, economical and fast.3. Renal index can be used as a indicator to evaluate the therapeutic efficacy of the treatment of Candida albicans infections. This kind of method is simple, economical, at the same time,has high sensitivity and good repeatability.