Study On The Apoptotic Effects OfⅠA PI3K/AKT Singaling Pathway On Leukemia HL-60 Cells Induced By Oleanolic Acid

Objective:To provide new clues for AML targeted therapy by researching the apoptotic effects ofⅠA PI3K/AKT singaling pathway on leukemia HL-60 cells induced by oleanolic acid.Methods:The growth-inhibiting effects of PI3K inhibitor(LY294002) on HL-60 cells were detected by MTT assay ; After being treated by drug, the morphological and structural changes of the cells were observed by transmission electro microscopy ; In HL-60 cells, after being treated by OA, the mRNA expressions of cav-1,pi3k,akt were measured by RT-PCR; CAV-1,AKT,p-AKT protein levels were measured by Western blot.Results:MTT experiment proved PI3K inhibito(rLY294002)has growth-inhibiting effects on HL-60 cells, which are enhanced with the increase of concentration in 24h; In HL-60 cells, after being treated by OA, apoptotic morphology changes are found by transmission electro microscopy, the mRNA expression of cav-1 is upregulated while the mRNA expressions of pi3k,akt are down regulated by RT-PCR test, CAV-1 protein level is increased and AKT,p-AKT protein levels are reduced by Western blot test.Conclusions:After being treated by 50μmol/L PI3K inhibito(rLY294002)in 24h, the growth-inhibiting effects on HL-60 cells are the best;OA can inhibit proliferation of HL-60 cells by PI3K/AKT Signaling pathway, the ratio of the P-AKT/AKT in medication group in 24h compared with LY294002 group is 1.18, explaining its inhibition effect is equivalent to 84.72% of LY294002 group; OA can inhibit PI3K/ AKT singaling pathway by upregulating the expression of CAV-1, resulting in inhibiting cell proliferation and promoting apoptosis in HL-60 cells.