Qi And Yin, Circulation Dispel The Cloud Medicine Of Diabetic Nephropathy In Rats No, Hcy

Purpose:Through the observation of the effect of the chinese herbal medicine on Nitric oxide and hcy and some other indexes of the diabetic rats ,investigate that the mechanism has a good curative effect on diabetic nephropathy (DN) with syndrome of blood stasis due to deficiency of both qi and yin. This research proved that the prescription offered a doable project for the cure of DN and preventing the earlier diabetic nephropathy on the chinese herbal medicineMaterial and method:1,material This research selected 77 Wistar male rats,which are the ordinary level ,four-monthold,150-180 grams. Formula: Radix astragali, ginseng, root of herbaceous peony, mountain cornus, fresh, leech, oriental water plantain, pinellia, mountain zhi. Western medicine: Luck Xin Pulley (business commodity name: Mongolian) 10mg/piece. The chain urea assists the rhzomorph (STZ): Near dissolves before in the 0.1mol/L, PH4.2 aseptic ju reason sour sodium buffer solution, matches 1% solutions.2, methode stablishes the diabetic rats model. To randomly divided into five groups:the Normal Contrasted Group,the Model Contrasted Group,the Ramipri Treated Group,the Chinese herbal medieine Treated Group,and the Chinese herbal medieine prevention group. The Model Contrasted Group,the Ramipri Treated Group,the Chinese herbal medieine Treated Group,and the Chinese herbal medieine prevention group were injected in peritoneal cavity one time by streptozotoe(45mg/kg).The prevention group to give the traditional Chinese medicine from the beginning, the treatment group after making the mold completes(1 month )gives the traditional Chinese medicine , the Ramipri Treated Group after making the mold completes gives western medicine luck Xin Pulley (Mongolian) for 8 weeks. 3, examination targetβ₂-MG, NO,HCY, PAI-1 of each mouse 12 weeks later.The extraction blood serum, the kidney,use the biochemistry method to examine microamout blood glucous.To examine kidney NO,HCY and the bloodβ₂-MG content, examine kidney PAI-1 with the immunity group reduction the expression. Uses HE to dye, observes the renal glomerulus basilar membrane and the department membrane change under the light microscope.Results:Compares with the Normal Contrasted Group, the Model Contrasted Group kidney NO,HCY level, the bloodβ₂-MG content and the PAI -1 expressions elevate obviously (P < 0. 01). The prompt Diabetic Nephropathy(DN)the model is successful; Compares with the the Model Contrasted Group, the Ramipri Treated Group,the Chinese herbal medieine Treated Group,and the Chinese herbal medieine prevention group NO,HCY level, bloodβ₂-MG and PAI - 1 expression obvious drop (P < 0. 05); Especially,the Chinese herbal medieine prevention group to drop obviously, and compares with the treatment group(P<0.05).Conclusion:This research proved that the prescription has a good curative effecton DN with syndrome of blood stasis due to deficiency of both qi and yin.It offered a doable project for the cure of DN. Especially, traditional Chinese medicine prevention group to diabetes nephrosis (DN) the protective function is more obviously, surpasses the treatment group and the masculine medicine group, explained that the profit raises, Chinese medicine not only to have the therapeutic action to DN more over also to have the important prevention function. Prompts the DN early time to take the traditional Chinese medicine to prevent can have the good effect.