Study On The Changes Of The Histomorphology And Content Of Hydroxyproline After Irradiated By γ-rays In Human Tendon

ObjectiveTo study the changes of the histomorphology and content of hydroxyproline that irradiated by 2.5 Mradγ-rays in human tendon.MetrhodsKeep the forty fresh human tendons freezed in-80℃for six weeks, and randomly divided into two group:The experimental group (group A) and control group (group B). Expose the group A that stored in dry ice inγ-rays until the absorbed dose reached to 2.5 Mrad. The tendons was stained with HE and VG methods, and then observed by common light microscope and transmission electron microscope respectively. Determine the content of hydroproline in tendon collagen hydrolysate by the high performance liquid chromatography with precolumn derivation.Results①The results of histomorphology observation reveals that compare to the control group, the gaps between collagen fiber bands in irradiation group were enlarged, incompact, disorder and some of them fractured. The electron microscope observation showed that the transverse striation of the collagen fibers became unclear or disappeared. The membrane of tendon cell dissolved, the cell nuclear disintegrated and the organelles reduced.②The content of hydroxyproline in the collagen hydrolysate of irradiation group was significantly higher than non-irradiation group under the same hydrolysis condition (P＜0.05).ConclusionThe morphology and structure of tendon changed significantly after 2.5 Mrad dose ofγ-rays irradiation. The content of hydroxyproline in collagen hydrolysate of irradiation group is significantly greater than that of non-irradiation group under the same hydrolysis condition.