Glutathione - The Synthesis Of Ferrocene And Its Interaction With Metallothionein,

The interaction between peptide and protein was investigated by electrochemical method. However, most of peptides or proteins are electro-inactive, so it is different to apply electrochemical method to study the interactions between peptides and proteins. In this study, an electrochemical method was employed for the determination of the interation between redox-inactive glutathione and metallothionein. The main works of the thesis are as follows:Reduced glutathionyl ferrocene (Fc-GSH, Fc:ferrocenoyl) and oxidized glutathionyl ferrocene (Fc-GSSG-Fc) were synthesized from ferrocene monocarboxylic acid, reduced glutathione (GSH), and oxidized glutathione (GSSG) by liquid-phase synthesis method with the yields of 12.5% and 46.0%, respectively. These compounds were characterized by IR spectrum, UV-vis spectrum,1H-NMR and mass spectrum.The interation between Fc-GSH and Zn7-MT was studied by electrochemical method. A pair of well-defined voltammetric peaks, with the anodic peak potential (Epa=0.218 V) and cathodic peak potential (Epc=0.154 V) were observed for Fc-GSH at Zn7-MT modified electrode (ΔEp=77 mV, ipa/ipc=1.13). However, the anodic and cathodic peak potentials were 0.205 V and 0.147 V, respectively, were observed for Fc-GSH at bare gold electrode (ΔEp'=58 mV, ipa'/ipc'=1.02). It indicates that Fc-GSH undergoes a reversible electron transfer reaction and a specific interaction between Fc-GSH and Zn7-MT, the binding constant and the binding ratio were 5.54±0.43×106 and 1.78±0.15, respectively.The interation between Fc-GSSG-Fc and Zn7-MT was studied by electrochemical methos as well. A pair of well-defined voltammetric peaks, with the anodic peak potential (Epa1=0.428 V) and cathodic peak potential (Epc1=0.351 V) were appeared for Fc-GSH at Zn7-MT modified electrode (ΔEp1=64 mV, ipa1/ipc1=1.03). Tthe anodic and cathodic peak potentials were 0.425 V and 0.353 V, respectively, were observed for Fc-GSH at bare gold electrode (ΔEp2=72 mV, ipa2/ipc2=0.96). It indicates that Fc-GSSG-Fc undergoes a quasi-reversible electron transfer reaction and a special interaction between Fc-GSSG-Fc and Zn7-MT, and Fc-GSSG-Fc can release zinc from Zn7-MT. It also demonstrates that there is an interaction between Fc-GSSG-Fc and Zn7-MT. The interactions of Fc-GSSG-Fc with Zn7-MT and apo-MT were investigated too. Inaddition, Zn2+forms a 1:1 complex with Fc-GSSG-Fc, this result is in good agreement with literature.