Rheumatoid Arthritis In Peripheral Blood Of Patients With Th17 Cells, Serum Il-17 And Il-6 Level Of Detection Of Disease Activity,

PartⅠ:Determination of Th17 cell subsets in patients of rheumatoid arthritis at different Period of activityObjictive:Detected by flow cytometry with rheumatoid arthritis peripheral the level of blood Th17cells.Methods:1.63 patients' which were diagnosed as RA and 15 healthy controls'Th17 cells were detected by flow cytometry.2.63 patients of RA were divided into high, medium and low activity group in accordance with DAS28 scores, which did multiple comparison with healthy control group. Preliminary study the level of Th17 cells in the determination of disease activity in RA patients with different levels of significance.Results:1.The level of Th17 cells of the patients which were diagnosed as RA is higher than healthy controls.2. The level of Th17 cells is higher in high activity group than in healthy control group. There is no significant difference between other groups.3. There is no significant difference between these groups which were divided by X-ray film of both hands.Conclusion:We can monitor patient's condition by testing Th17 cells in peripheral blood from patients with flow cytometry, and evaluate the severity of disease activity of patients. PartⅡ:The expression of IL-17 and IL-6 in serum of patients with rheumatoid arthritis by CBAObjective:Analyze the significance of IL-17 and IL-6 in various activities period of patients with RA by detecting their expression in serum of patients.Methods:1. Determine IL-17 and IL-6 in serum of 63 RA patients and 18 healthy controls by Cytometric Bead Array(CBA).2.63 patients of RA were divided into high, medium and low activity group in accordance with DAS28 scores, which did multiple comparison of their expression of IL-17 and IL-6 with healthy control group.Results:1. The level of IL-17 and IL-6 of the patients which were diagnosed as RA is higher than healthy controls.2. The level of IL-17 is higher in high activity group than in healthy control group. There is no significant difference between other groups. There is no significant difference in IL-6 between all groups.3. There is no significant difference between these groups which were divided by X-ray film of both hands.Conclusion:There is significant difference in serum of IL-17 and IL-6 between RA patients and healthy people. Join in detection of Th17 cells in RA may predict disease progression and determine disease activity by these biomarkers.