Psoralen Particles Induced By Cisplatin In Mouse Ovarian Injury In Rats

Chemotherapy as the main anti-cancer treatment, which can cause serious damage to the female ovarian structure and function, and lead to premature ovarian failure and related infertility. Therefore, how to treat the tumors while preserving women's ovarian function is a huge medical need, as well as is an important topic worthy of study. Western medicine generally used estrogen replacement therapy (hormone replacement therapy, HRT), but increased risk of estrogen-dependent cancer. Phytoestrogens are a bi-directional regulatory role, avoiding the high side of the HRT and are are the more secure ingredients of treatment. Based on traditional Chinese medicine theory, our laboratory has screened a variety of Zishen Yijing to reconcile qi and blood Chinese medicine, Found that these Chinese medicines have been the role of plant estrogen-like. Psoralen is one of them.In recent years, the researches on psoralea alleviating the side effects after chemotherapy were increasingly more, mainly for some renal toxicity, immune function degradation, etc. Little research has been conducted in applying Psoralen to protect cisplatin induced ovarian dysfunction. Based on theoretical explanation and experimental activities on applying phytoestrogen to treat gynaecopathia in our laboratory, Psoralen Particles was selected to act on ovary cisplatin- induced. This study may observed whether Psoralen Particles had the protection, and provide theoretical guidance and guidance for clinical use of drugs in the future.The topic for a two-class research of protective effects of Psoralen on cisplatin induced ovarian dysfunction:1 The overall level of researchCisplatin is a cytotoxic non-specific drug, with strong broad-spectrum anti-cancer effects. At the same time it has a strong liver and kidney toxicity. Protective effect of psoralen is reflected in its Entirety.1.1 The change of morphology and body weight in cisplatin injury mice with gavaging different doses of psoralen ParticlesIntraperitoneal injection of cisplatin in mice, then the mice showed dull colour coat and erected-hair, dead-eyes, lying curled, decline in food and weight loss. Stop injection of cisplatin, weight has gone up, but clear differences between the symptoms improved in each group. Particles of different doses of psoralen gavage the mice, they have a good recovery of morphology and body weight in varying degrees.1.2 The change of Liver, spleen, kidney, device quality in cisplatin injury mice with gavaging different doses of psoralen ParticlesThere were many studies on cisplatin on liver and kidney toxicity. Intraperitoneal injection of cisplatin result in liver, kidney, spleen injury, such as the decline in the quality, organ coefficient reduced and so on. After psoralen particles gavage, the mice of the organ coefficient values are higher than the model group, especially kidney coefficient. This also shows that the psoralen in the role of nourishing and protecting kidney.1.3 The change of the quality and morphology of ovarian and uterine in cisplatin injury mice with gavaging different doses of psoralen ParticlesThe clinical impact of Cisplatin on female genital mutilation is like in premature ovarian failure, mainly for the:(1) Secretion function of mouse uterus and ovaries have gone down. Mice ovary and uterus quality and coefficient decreased. (2) HE staining can be observed in mice ovarian tissue after injury an increase in atretic follicles. HE staining can be observed in mice ovarian tissue after injury an increase in atretic follicles. Immunohistochemistry can be seen the expression of aromatase decrease.Psoralen large, medium and small-dose group mice with different ranges of regulating hormone levels return to normal, and to improve the quality and coefficient of ovary and uterus. And the increase in the number of developing follicles to enhance the expression of aromatase, thereby contributing to the substrate converted to estrogen.2 The cell level of research2.1 Effects on the morphology of ovarian granulosa cells of pharmacological serum at different timeAfter the effects of cis-platinum, cell morphology changed:cell disruption, poor adhesion, cell concave, perinuclear vacuoles appearance, and Organelles within the cytoplasm disappearance. Granulosa cells of psoralen pharmacological serogroups were relatively regular, and cell debris was less.2.2 Effects on the amount of ovarian granulosa cells of pharmacological serum at different timeComparison of the measured absorbance value with CCK-8 cell counting method before Cis-platinum damage, and the measured absorbance value with MTT cell counting method after Cis-platinum damage. Cisplatin before, from the absorbance values reflect the number of ovarian granulosa cells can be used as the base. Cis-platinum after, the value changes that have occurred indirectly reflected the protective effect of ovarian granulosa cells of psoralen in serum pharmacology. This conclusion is mainly manifested in the the number of cells of psoralen group more than the model group.2.3 Effects on the estrogen secretion of pharmacological serum at different timeGranulosa cell aromatase activity is low, and the lower substrate concentration. After adding estrogen follicles (FSH) stimulation, aromatase activity was rapidly increased, then Catalytic substrate into a large number of estrogens. Over time, the amount of estrogen secretion gradually decreased. Psoralen pharmacological serum can slowly and long-term increase aromatase activity, so that the amount of estrogen at a certain range within a certain period of time maintained.To sum up, psoralen particles at a certain dose showed good protective effect, on cisplatin-induced ovarian damage. This conclusion may provide theoretical guidance for clinical use of drugs in the future.