Expression Of Snail And E-cadherin In Bladder Cancer And Related Research

Objective:1. To study the expression of Snail and E-cadherin in urothelial bladder cancer, and the difference between pathological grades and stages.2. To verify the corelation between expression of Snail and E-cadherin, and investigate the effect on the prognosis.3. To provide basis for research.Material and Method:67 urothelial bladder cancer specimens were studied, all of which obtained during 2004-2007 by TUR or cystectomy. Clinic data was achieved by review of case history and telephone visit. Slices were cut from paraffin blocks with help of Pathology Dept, Huashan Hospital, and then used for immunochemistry coloration. Grouping specimens by pathologic grade(Gl,G2,G3),clinic stage(Ta-1,T2-4) and clinic progress data, then statistics analysis was performed with SPSS software between groups.Results:67 urothelial bladder cancer specimens were investigated, of which 54 from male,13 from female,23 of Gl grade,25 of G2 grade,19 of G3 grade,49 of TL stage,18 of TH stage.6 normal bladder tissues were investigated by IHC. The expression of E-cadherin decreases when grade is high, statistics analysis shows significant difference between G1,G2,G3(G1*G2 p=0.011,G2*G3 p=0.009, G1*G3 p<0.001), and higher expression in TH group than TL (p=0.009)。Inversely, Snail expresses higher when grade is higher, statistics analysis shows significant difference between G1,G2,G3(G1*G2 p=0.008 G2*G3 p=0.023 G1*G3 p<0.001), and expresses higher in TH group than TL (p<0.001). Spearman test indicates that Snail has a reverse corelation with E-cadherin expression (r=-0.733, p<0.001). Group of specimens from patients that underwent muscle invasion or metastasis after TUR in 2 year has higher expression of Snail(p=0.021),and lower expression of E-cadherin(p=0.032) than group without invasion or metastasis during the same time duration.Conclusion:E-cadherin expresses lower, while Snail expresses higher, when clinic stage and pathologic grade is higher, and the result that Snail reversely express with E—cadherin in urothelial bladder cancer, suggests Snail would participate regulation of E-cadherin, which sustain the premier researches。The low expression of E-cadherin, high expression of Snail of invasive and metastasis group to some extent suggests the prognosis effect of the two factors.