Alpha-lipoic Acid To Combat Type 2 Diabetes Animal Models Of Pancreatic Injury

[Objective] To establish an appropriate animal model of type 2 diabetic pancreatic and hepatic lesions and evaluate the efficacy and potential risk of lipoic acid on these pathologic changes.[Methods] First part:GK and Wistar rats(n=32/each) were randomly divided into two group separately, one of each strain' group were fed with SW, the other with normal water. Some animals were sarcrificed on 2,4,6,10 wk respectively. The clinical chemistry assessments were conducted. Pathological changes of liver, pancreas, kidney and heart were observed.Second part:GK rats, were randomly divided into six groups:one negative group, one positive group, four LA administered groups. Five groups (except for the negative group) were fed with SW. LA was administered by oral gavage every day for 9 weeks. The amount is respectively:25,50,75,100 (mg/kg).The body weight and FBG was examined every week. At the end of the test, all rats were sacrificed, clinical chemistry assessments were conducted and organs were stained with haematoxylin and eosin (besides, pancreas was stained acoording to the Mallory method), all the sample slides were examined microscopically.[Results] First part:(1)After fed with SW, for the WS group, the body weight and serum TG gradually increased.The organ indexes (except for heart) gradually decreased. Pathological lesions of liver and pancreas gradully increased.(2) After fed with SW, for the GS group the body weight gradually decreased, fasting blood glucose level fluctuation increased, serun TG gradually increased, serum Glu initially decreased then gradually increased.The pancreatic and hepatic organ indexes and pathological lesions gradually increased.Second part:After fed with SW and/or LA, the body weight showed no changes. Compared with G group, the FBG of GS group gradually increased, serum ALT and pancreatic pathological lesions significantly increased.Compared with GS group, along with the escalation of LA dose, four LA administered groups'ALT and AST gradually increased, TG and pancreatic pathological lesions gradually decreased. But the pathological lesions of liver, heart and kidney gradually increased.[Conclusion] First part:(1)It was not successful to establish animal model of type 2 diabetic pancreatic and hepatic lesions by the way Wistar rats were fed with 10 wk SW. But it was successful to establish animal model of type 2 diabetic pancreatic and hepatic lesions by the way GK rats were fed with 10 wk SW. (2) The adaptive ability did not disappeared for GK rats in their beginning of disease. (3) After fed with SW, GK rats' pancreatic pathological lesions occurred earlier than hepatic lesions.Second part:25～100mg/kg LA can dose-dependently improve the pathological lesions of type 2 diabetic rats, and decrease the serum TG. But, aslo dose-dependently aggravated the pathological lesions of liver, heart and kidney.