| 1. PurposeLearning and memory is the highest performance of brains. It is also the foundation to develop human society activities. All of the things like study grades, the efficiency of works, and dealing with daily things are involved in the matters of learning and memory. People are stressed from working in this highly developing society, which cause physical and mental fatigue and fatigue lead to learning and memory decline. Therefore, how to improve the body function and to recover from the situation of fatigue is the most import thing.This study is based on previous study granted by National Natural Science Foundation of China in the subject of "The mechanism on herbs that regulate and tonify Qi eliminating exercise-induced fatigue" This research show that regulate liver Qi herbs are the better for restoring the memory decline caused from exercise-induced fatigue. Based on this concept, researchers used regulate liver Qi herbs combining with the Chinese herbal formula of nourishing the kidney and heart to treat sleep deprived and exercise-induced fatigue rats. And by using Immunohistochemistry, Western Blot, Real time PCR to exam and analysis CREB and PKA in rats brain.2. Methods and Results(1) Model evaluationOur study shows that there's obvious weight loss in model group. In behavior exams, there are significant difference between model group and normal group. Besides, there are loose stools, scattered hair, irritability and mutual attack showed in model group. Serum lactate dehydrogenase (LDH) results are also significantly higher in model group then in normal group. These results indicate successful fatigue modeling. The three groups that been treated with formulas have less weight loss then model group; have better performance in balance examination, and have lower scales of LDH then model group. Study shows Chinese medicine has certain effects on fatigue resistance. Among all, combined formula group has the best out come, followed by regulate liver group and the last is tonify kidney and nourish heart group.(2) Y MazeIn Y maze memory assessments, model group have the worst memory. Among all herbal treating groups, combined formula group has the best out come, followed by regulate liver group and the last is tonify kidney and nourish heart group.(3) CREB and PKACREB and PKA are both important neurotransmitters in brain, which forms long term memory.In Immunohistochemistry experiments, the expression level of CREB and PKA in hippocampus CA1, CA2, CA3 and DG regions of model group are poor, unclear cell staining and less positive cells. In all herbal treating groups, CREB and PKA are well expressed in hippocampus CA1, CA2, CA3 and DG regions, cell nuclear are clearly stained. Among all herbal treating groups, combined formula group has the most positive cell counts and expression, followed by regulate liver group and the last is tonify kidney and nourish heart group.In Western Blot, PKA protein level is significantly lower in model group. Among all herbal treating groups, combined formula group has the highest level, followed by regulate liver group and the last is tonify kidney and nourish heart group.In Real time PCR, CREB mRNA level is significantly lower in model group. Among all herbal treating groups, combined formula group has the highest level, followed by regulate liver group and the last is tonify kidney and nourish heart group.SummaryIn this study, we use swimming and sleep deprivation to simulate decline memory by modern society, study behavioral tests, and analysis CREB and PKA levels by using Immunohistochemistry, Western Blot and Real time PCR to distinguish the effects of regulate liver Qi formula, tonify kidney and nourish heart formula and combined formula working on fatigue rats. By experiments, we realize there are memory improvements by using Chinese medicine. There are lots of factors causing fatigue; most studies are restricted by epistemology and methodology, and use one method to treat it, thus, lack of fully coverage reports. By using formulas to exam TCM syndrome can help explain the relations among heart, liver and kidney and the improvement of memory loss induced by fatigue. |
PDF Full Text Request