Licorice Flavonoid Gypenosides Arachidonic Acid Pathway

Licorice is the most commonly used clinical application of Chinese medicine,and Gynostemma is a growing emphasis on the discovery of modern Chinese medicine. The Flavonoids contained by former and the saponins contained by later are belong to the two main active ingredients. Recently found that they all have anti-tumor, anti-inflammatory, antioxidant and other pharmacological effects. Arachidonic acid metabolites are extensively involved in the pathological process. Whether Gypenosides flavonoids and metabolism of arachidonic acid through the intervention while the performance of the pharmacological pathway and what is the mechanism. In this paper,we have used LPS to stimulate the cells of RAW264.7 to construction system, and observed the effects of some tools medicine and Flavonoids, gypenosides to the pathway. We expect to Test and verify the reslts with lipid science technology group.The main findings are as follows:(1) LPS can Induced the PLA2 of RAW264.7 expression increased. Aspirin (COX inhibitor) and Luteolin (15-lipoxygenase inhibitor) show a clear antagonism (P<0.05). Other tools Medicine. Yet other tool medicines for example TMS(selective inhibitor of CYP450), Caffeic acid (LOX inhibitor), Thio-PC (sPLA2 selective inhibitor), CAY10397 (15-PGDH selective inhibitor), baicalein (12-LOX inhibitor), Bestatin (LTA4 hydrolase inhibitor), SC-560 (COX-1 selective inhibitor) and licorice flavonoids and gypenosides content on PLA2 compared with the groups of LPS, the resoultis not statistically significant. However, this trend shows that lmg/mL and 0.5mg/mL of the flavonoids can inhibit the activity of PLA2 but 0.25mg/mL the flavonoids could promote the activity of PLA2.(2) LPS treatment led RAW 264.7 cells increased expression of COX-2. Low-dose aspirin(COX inhibitor), low-dose caffeic acid, low-dose CAY10397, low-dose SC-560 and a small dose of Thio-PC have shown that the expression of COX-2 antagonist increased. Although other tools of drug groups have different degrees of antagonism, however the difference was not a significant compared with the group of LPS.(3) With LPS stimulation, the P450 of RAW 264.7 cells was up. In addition to TMS (CYP450 selective inhibitors) against the role of LPS (P<0.05), Other reagents under the experimental conditions have not obtained statistically significant positive results (P>0.05).(4) In our experimental conditions, the test tool drugs (Aspirin, SC-560, Bestatin, Thio-PC, CAY10397) are shown to inhibit the increase in LPS stimulated PGE2 release (P<0.05),and different concentrations of the flavonoids and gypenosides also showed similar results gypenosides.These results show that gypenosides and flavonoids may inhibit the release of cyclooxygenase to Inhibit of PGE2 release and the inactivation of enzymes with unrelated PLA2 and PGs.