Adam33 Gene Expression In Chronic Obstructive Pulmonary Disease And Its Significance

Objectives:The ADAM33 gene mapping in 20 chromosome short arm 20p13, belongs to the ADAM gene ultra family. Through the gene chip research discovery, ADAM33 mainly in the brain organization, the spinal cord, the lung organization, the placenta, the spleen and the vein expresses, expresses the level in other organizations to be low. In the lung organization ADAM33 most important in the smooth muscle cell, the muscle fiber cell, becomes the ciliary cell to express, but in the epithelial cell, the T lymphocyte as well as the inflammatory cell has not seen the expression. The COPD condition development's key is the airway heavy new moon.This research is for the purpose of examining ADAM33 mRNA to express and to analyze its significance in the chronic obstructive pulmornary disease.Methods:part one: Uses semi-quantitative RT-PCR the method to examine in the big mouse lung organization the ADAM33mRNA expression level, and carries on statistics analysis to the obtained data;part two: Uses semi-quantitative RT-PCR the method to examine in the person venous blood the ADAM33mRNA expression level, and carries on statistics analysis to the obtained data.Results : part one:ADAM33mRNA expresses the masculine gender 10 examples in the COPD model big mouse lung organization, the masculine gender rate is 83.3%, in the normal group expresses the masculine gender 1 example, the masculine gender rate is 12.5%; Expresses the level in the COPD model big mouse for (1.0035±0.29), expresses the level in the normal big mouse for (0.6130±0.20). The model group expresses the level to be higher than the control group obviously, has statistics significance;part two: In 20 example AECOPD patient venous blood, ADAM33 mRNA the expression masculine gender 19 examples, the masculine gender rate is 95%, expression level for (0.9185±0.25); in 10 example COPD remission patient venous blood, ADAM33 the mRNA expression masculine gender7 examples, the masculine gender rate is 70%, expression level for (0.58420±0.20); in 20 example normal person venous blood, ADAM33 the mRNA expression masculine gender 6 examples, the masculine gender rate is 30%, expression level for (0.3486±0.14). The COPD masculine gender expression rate and the expression level are higher than obviously illuminate the group normally, has statistics significance. COPD acute aggravation time ADAM33 the mRNA masculine gender expression rate and expresses the water to be higher than the stabilization period equally, has statistics significance. COPD patient ADAM33 the mRNA expression level and the FEV1/FVC value assume the negative straight line correlation.Conclusions: ADAM33mRNA the masculine expression rate and the expression level is higher than the normal control group in the COPD model big mouse lung organization, the masculine expression rate and expresses the water in the COPD patient venous blood also obviously to be higher than the normal person control group equally, COPD acute aggravation time ADAM33 the mRNA masculine gender expression rate and expresses the water to be higher than the stabilization period equally, has statistics significance, and COPD patient ADAM33 the mRNA expression level and the FEV1/FVC value assume the negative straight line correlation, extrapolated that the ADAM33mRNA expression upward possible and the chronic blocking lung disease condition are related.