Qi And Yin, Blood Stasis And Huazhuo Chinese Medicine Diabetic Rat Kidney Ang Ii, Et

Purpose: The observation profit gas raises removes extravasted blood the muddy traditional Chinese medicine cloudy to diabetes big mouse kidney angiotensinⅡ(AngⅡ) endothelin (ET)as well as the plasminogen activator inhibitor-1 (PAI-1 influence, further discusses the traditional Chinese medicine to diabetes big mouse kidney's protective function mechanism.Material and method: 1st, material health Wistar big mouse 77, are the ordinary level, the male gender, the body weight 150-180 grams. Formula: Radix astragali, ginseng, root of herbaceous peony, mountain cornus, fresh, leech, oriental water plantain, pinellia, mountain zhi. Western medicine: Luck Xin Pulley (business commodity name: Mongolian) 10mg/piece. The chain urea assists the rhzomorph (STZ): Near dissolves before in the 0.1mol/L, PH4.2 aseptic ju reason sour sodium buffer solution, matches 1% solutions.2nd, method establishes the big mouse diabetes (DM) the animal model. The sugar feed feeds big mouse one month and the abdominal cavity injection chain urea through Gao Zhigao assists the rhzomorph (STZ) the 45mg/kg induction to prepare the DM animal model, and supposes the normal control group, the model group, the treatment group, the masculine medicine group, the prevention group. The prevention group while gives Gao Zhigao the sugar feed to give the traditional Chinese medicine to fill the stomach immediately, the treatment group after making the mold completes(l month )gives the traditional Chinese medicine to fill the stomach treatment, the masculine medicine group after making the mold completes gives western medicine luck Xin Pulley (Mongolian) to fill the stomach treatment Treats for 8 weeks. 3rd, examination targetβ2-MG in blood, AngⅡ,ET ,PAI-1. each group of big mouse 12 weeks later executes, the extraction blood serum, the kidney, with the biochemistry law examination blood sugar, emit the immunization to examine kidney AngⅡ, The ET content and the bloodβ2-MG content, examine kidney PAI-1 with the immunity group reduction the expression. Uses HE to dye, observes the renal glomerulus basilar membrane and the department membrane change under the light microscope.Results: Compares with the normal control group, model group big mouse kidney AngⅡ, The ET level, the bloodβ2-MG content and the PAI -1 expressions elevate obviously (P < 0.01) The prompt diabetes nephrosis (DN) the model is successful; Compares with the model group, prevention group, treatment group, masculine medicine group kidney AngⅡ, ET level, bloodβ2-MG content and PAI -1 expression obvious drop (P < 0. 05); Specially prevents the group to drop obviously, and compares with the treatment group (P < 0. 05).Conclusion: The profit gas raises cloudy, removes extravasted blood the muddy traditional Chinese medicine to have the therapeutic action to diabetes big mouse kidney pathological change, its effect and the masculine medicine group are the same. Specially traditional Chinese medicine prevention group to diabetes nephrosis (DN) the protective function is more obvious, surpasses the treatment group and the masculine medicine group, explained that the profit gas raises cloudy, removes extravasted blood the muddy traditional Chinese medicine not only to have the therapeutic action to DN moreover also to have the important prevention function. Prompts the DN early time to take the traditional Chinese medicine to prevent can have the good effect.