Experimental Research On Salvia Intervention In Alzheimer's Disease Rat Model Of Immune Inflammatory Injury

Alzheimers disease(AD),also named as senile dementia,is an acquired syndrome of intelligence injury because of cerebral disfunctions,It is a retrograde disease of nerve system related to loss of memory and hypofunction of higher cognition.TCM thinks the origin is consumptive disease of five viscera and the superficiality is the stagnation of blood and phlegm in the body.Basing on of Danshen' function of promoting blood circulation by removing blood stasis,in this experiment study we investigate mechanism of Danshen in treatment of AD.Purpose:To study the function of Danshen to immune inflammation injury of Aβmodel AD Wister rats,to observe the effect of Danshen to inflammatory bell genesIL-6,TNF-αof model Wister rats,and to discuss the effective mechanism of Danshen so as to provide scientific evidence for TCM to treate AD.Material and method:The rat AD models were set up by Aβ-amyloid protein injection into the Hippocampus Wister rats,for them traditional Chinese medicine Danshen,Western medicine indomethacin,and saline were administered. After four weeks,effect of drugs on learning and memory in rats is observed by the water maze experiment:ELISA method was used to detect expressions of cytokines serum IL-6,TNF-αin all groups.Results:In the water maze experiment,compared with pre-administration rats, the incubation and escape times of Wister rats given Chinese medicine and Western medicine had been shortened,lt showed that the two drugs for AβWister rats can improve learning and memory ability.In cytokines detection,compared with sham operation group,the IL-6,TNF-αin model group had higher level of expression.It showed that injection of Aβamyloid protein could make secretions of IL-6,TNF-αin serum significantly increase.Compared with model group, IL-6,TNF-αsecretion in the Chinese medicine group and western medicine group reduced.It revealed that both Chinese medicine and western medicine could inhibit expression of IL-6,TNF-αin the AβWister rats serum.Conclusion:1 The escaping latency of AD model Wister rats established by using Aβ-amyloid protein injection in to the Hippocampus is prolonged,learning and memory decline expression of cytokines increased in serum IL-6,TNF-αis increased,which may be one of the pathological changes in Wister rat AD models.2 Danshen can improve learning and memory of rat models and inhibit the expression of cytokines IL-6,TNF-αin AD serum.It shows of inhibition to the immune inflammatory injury of AD model Wister rats may be one of the mechanisms...