| Background Altered expression and structure of glycolsacchafide were associated with tumor development and metastasis.Carbohydrate structures with a 3'-sulfoβGal linkage have been thought to play an important role in many biological processes, such as cell adhesion,inflammation,and cancer metastasis.In the current study,we investigated the significance of 3'-sulfo- le~a antigen in gastric carcinoma and colorectal carcinoma.Methods Serum 3'-sulfo- le~a antigen expression was analyzed in 108 gastric carcinoma patients and 78 colorectal carcinoma patients by Enzyme-linked immunosorbent assay.For comparison,levels of serum 3'-sulfo- le~a antigen were also analyzed in 74 healthy volunteers as the control.Furthermore,we investigated the biological function of 3'-sulfo- le~a epitope by GP3ST targeted RNAi.Results The mean levels of serum 3'-sulfo- le~a antigen epitope were lower in colorectal carcinoma(8.001±2.493μg/mL),and higher in gastric carcinoma (3.506±5.204μg/mL)(p＜0.001 ) than that in the healthy control(9.104±5.159μg/mL) .Depth of invasion(p=0.018),lymph node metastasis(p=0.002) and tumor cell differentiation(p=0.031) are noted to be significantly correlated with the expression of this epitope in gastric carcinoma.On the contrary,significantly decreased expressions of 3'-sulfo- le~a antigen were detected in colorectal carcinoma with lymph node metastasis(P=0.044)and venous invasion(P＜0.001) than without metastasis. After RNAi transfection,a significant decrease in adhesion to sL-selectin and HUVEC of SCG7901 were observed.Conclusions These results suggested that 3'-sulfo- le~a epitope was involved in tumor metastasis process.Changes of serum 3'-sulfo- le~a antigen provide important prognostic information in patients with primary gastric or colorectal cancer,which might be useful as a predictive marker in the following up to evaluate prognosis,the progress of the cancer,and to monitor the therapeutic effect of chemotherapy. |
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