| Objective:This experiment is to observe the effects of repetitive freeze-thawing treatment before allograft tendon transplantation on histology and biomechanics of rabbit Achilles tendon.So we can understand the effect of different number of times repetitive freeze-thawing treatment to Achilles tendon and this can offer some theoretic directions to clinical application of allograft tendon transplantation.Method:33 rabbit Achilles tendons were harvested with calcaneus bone block,0.6 cm×0.4 cm,and exposed to 60Co irradiation for 68 minutes at 25 kGy.The tendons were divided randomly into three groups,stored at -80℃,and thawed at room temperature for 1(control group),3 and 10 times(experimental group).In addition,seven tendons were selected randomly from each group for biomechanical determination and undertake statistical analysis,and 4 tendons for histological observation.Result:Histological observation showed that with increasing freeze-thawing,the ranks of tendon bundles and collagen fibrils become disordered until ruptured;cell disrupted,and apparent tendon bundle gaps were caused by ice crystal formation.Biomechanics results showed that the values of maximum load,maximum load energy,and maximum stress were significantly decreased with increasing freeze-thawing times(P＜0.05),while elastic modulus and energy density were slightly decreased.Conclusion:Repetitive freeze-thawing has apparent histological and biomechanical effects on rabbit Achilles tendon before allograft tendon transplantation,such as cell disruption,collagen fibrils disorder,ultimately biomechanical reduction. |
PDF Full Text Request