Ugyen Capsule Quality Standards For The Establishment Of Its Pharmacodynamic Study

? Wujin capsule is an civil experience Prescription, it is composed of cuttlebone, Endothelium Corneum Gigeriae Galli, Fritillaria thunbergii , costus root and Chinese goldthread, it can neutralize gastric acid to relieve pain, stanch the blood, and it has obvious effect for gastric ulcer, Duodenal ulcer, chronic gastritis. The prescription was originally set up to take the form of pulvis, but poor adaptability, dose, and not easy to carry, storage.? In the preparation technology research, We adopted L9(34) orthogonal experiment extraction process to optimize fritillaria thunbergii, costus root and Chinese goldthread extraction technology. By extractable amounts of berberine from Chinese goldthread, we investigated different moisture content, decoction time and times, then optimized water extraction process condition. To establish the optimal conditions are as follows: decoction three times, a 12-fold increase in water, decoction 1hour.? In the study of quality standards, we formulated the identification methods of cuttlebone, Chinese goldthread, costus root by acid-base titration and thin-layer chromatography. According to the composition of cuttlebone, we added diluent HCl in order to produce gas, and we deduced from the gas which material it is. For Chinese goldthread, we place standardization of Wujin capsule on a same silica gel G thin layer plate and put into developing solvent, development, take out it, airing, put thin layer plate into ultra-violet light box, we can see same color speck for related to medicinal materials and control article. For costus root, we extract Wujin capsule and costus root separately, then put on the same silica gel G thin layer plate and put into developing solvent, development, take out it, airing, put thin layer plate into ultra-violet light box, we can see same color speck for related to medicinal materials and control article. ? Additionally, to establish the drug ingredients CaCO3, total nitrogen content and the content of berberine content determination method for index. For cuttlebone with main ingredients CaCO3, through to the cuttlebone acid-base titration Ca2+ ions quantitatively, we can calculate in the content of CaCO3. For Endothelium Corneum Gigeriae Galli, through Kjeldahl method, we measure the content of nitrogen in the endothelium. There are relatively large content of berberine in Chinese goldthread, so we assayed the content of berberine by means of HPLC. Through verified test, Acid-base titration, Kjeldahl nitrogen determination. After verification testing, acid-base titration, Kjeldahl nitrogen determination, HPLC method repeatability, good stability and they can be used as the quality of Wujin detection methods. We require qualified drugs are as follows: a Wujin capsule of calcium carbonate content of between 45% ~ 55%, total nitrogen content of between 15% ~ 20%,berberine content of 0.5 mg·g-1 ~ 0.60 mg·g-1.? In the research of pharmacodynamics, we carried out model experiments in mice through water stress method, dose regimens, validated the effects of Wujin capsules in the treatment and prevention of acute gastritis and stress ulcer. Finally, we concluded that Wujin capsules have not obvious effect on stress-induced acute gastritis and ulcer treatment and prevention.