Ape, Vegf Expression In Cervical Carcinoma And Its Relationship With Mvd,

Objective To investigate the expression of apurinic/apyrimidinic endonuclase (APE) and vascular endothelial growth factor (VEGF) in cervical carcinoma, and also evaluate their role in tumor initiation, development and angiogenesis.Methods Sixty-one cases of cervical carcinoma, twenty-four cases of cervical intraepithelial neoplasia and ten cases of normal cervical tissues as control were collected from the affiliated hospital of medical college QingDao university from the year 2006 to 2007. All the cases was confirmed by pathological diagnosis. MVD and expression of APE and VEGF were detected by immunohistochemistry method in 61 cases of cervical carcinoma, 24 cases of cervical intraepithelial neoplasia and 10 cases of normal cervical tissue . The espression of APE , VEGF and MVD were analyzed in different clinical pathological indicators of cervical carcinoma , and their relationship was also analyzed. All statistical analyses were carried out with spssl3.0 software. The continuous variables were analyzed with student test , Fisher's exact test and Spearman's rank correlation coefficient. P<0.05 was considered statistically significant.Results (1)APE protein was positive staining in cervical carcinoma and predominantly localized in the nucleus of tumor cells. Fifty-three of sixty-one cervical carcinomas (86.9%) were determined to demonstrate high levels of APE expression , whereas the remaining 8 showed low expression of APE. Thirteen of twenty-four cervical intraepithelial neoplasia showed high expression of APE (54.2%). One case of normal cervical tissue showed high expression of APE (10.0%). The protein level of APE in cervical carcinoma was significantly higher than that of CIN and normal cervical tissue (P<0.05), and that of CIN was higher than normal cervical tissue(P<0.05). (2) VEGF protein was positive staining in the cytoplasm and cell membrane.CD34 was positive staining in the cytoplasm of vascular endothelial cell. The percentage of the expression of VEGF increased by turns in normal cervical tissue , CIN and cervical carcinoma, and the rate was 0% , 41.7% and 65.6% respectively. It had significant difference between cervical carcinoma and CIN and normal cervical tissue (P<0.05) , and that was also between CIN and normal cervical tissue (P<0.05).The count of MVD increased by turns in normal cervical tissue , CIN and cervical carcinoma, and that was 4.8±1.2 ,19.5±6.3 , 41.5±2.9 respectively. Through the comparison between the two of them , the difference had statistical significance(P<0.05). (3) Spearman's rank correlation coefficient showed that the positive rates of APE with that of VEGF and MVD showed significant correlations (r=0.795,P<0.05;r=0.607,P<0.05). The positive rates of VEGF and MVD showed significant correlations (r=0.651,P<0.05) .(4) There was a relationship between the expression of APE and pathological grading and clinical staging (P<0.05). So it was between the expression of VEGF and MVD and pathological type(P<0.05).Conclusions (1) APE plays an important role in the formation and development of cervical carcinoma, and can be used as the sign to judge the malignant degree of cervical carcinoma. (2) VEGF participated in the formation , development and the invasion of cervical carcinoma through inducing the angiogenesis of the neoplasm. (3) APE plays an important role in the angiogenesis of cervical carcinoma through regulating the expression of VEGF, then participates in the formation , development and invasion, but the precise mechanism is not clear now. (4) Detecting to APE and VEGF together may have an effect in the diagnosis , treatment and judging the prognosis of cervical carcinoma . APE may become a new target mole to resist the angiogenesis of cervical carcinoma.