Deep Cervical Muscle Spindle Densities And Nt-3, Of Trk C Expression In The Dorsal Root Ganglion Degenerative Cervical Spine Instability,

Objective:To observe the density of muscle spindles in human cervical deep muscles and immunohistochemical localization of Neutrophin-3 and Tyrosine kinase C in human dorsal root ganglion at different age grades,find their relations to degenerated cervical spinal instability.Methods:Took 12 individuals ranging from 0 to 81 years old as the study example, we used the quantity area ratio to show the density of musculus longus colli and musculus splenius capitis and immunohistochemical methods to manifest the expression of the NT-3 and Trk C in human dorsal root ganglions at different age grades.The results were analysised by t test.Results:(1) Muscle spindle density analysis:Muscle spindle density of infant group was highest among the three groups(p<0.01),but there was no significant difference between juve group and gerontal group(p>0.05).(2) NT-3 immunohistochemistry results analysis:dyeing intensity which was demonstrated with light absorbance of juve group was the highest,whilst which of infant group was higher than gerontal group's,and the differences among the three groups were all significant(p<0.01).(3) Trk C immunohistochemistry results analysis:dyeing intensity which was demonstrated with light absorbance of juve group was the highest,whilst which of infant group was higher than gerontal group's,and the differences among the three groups were all significant(p<0.01).Conclusions:On the course of degenerated cervical spinal instability,the diversity of the muscle spindle density may not play roles in it,maybe the neuroelectricity conduction function of the spindle is the key point.And the immunohistochemistry results of the expression of NT-3 and Trk C in human dorsal root ganglion at different age groups exactly accord with the condition of the cervical vertebral stability and motion function,which show that expression of NT-3 and Trk C in human dorsal root ganglion must be relevant with cervical spinal stability.