Meridians And Kidneys, And Compound On Glomerular Mesangial Cells Express Tgf-beta, The Fn And Ln Affect Experimental Research

GS(glomerular sclerosis) is the final common result of chronic glomerular injuries affected by all kinds of reasons,and it is leading to the end-stage of chronic renal failure occurred by a variety of renal progressive diseases.Therefore,the extent of GS indicates the degree damaged to the renal function and determines the prognosis on the chronic nephropathic patients.So its genetic and developmental mechanism and prevention and cure are now catching more and more attention both to domestic and overseas researchers.GS is characterized by the excessive accumulation and deposition of ECM(extra cellular matrix) and proliferation of GMCs(glomerular basement membrane), furthermore occurs damages onto structure of renal cells and functions,and decreasing of glomerular filtration rate,and eventually renal failure.Glomerular sclerosis derives from a very low dynamic process,which involves the complicated interaction between cells,cytokines and ECM,and finally the come-off is a great amount of ECM depositing in glomerular capsule.Currently,there are a lot of studies on the increase of ECM proteins synthesis in glomerular sclerosis,and it is adequate.From the point view of morphological,glomerular sclerosis arises from degradation imbalanced and excessive accumulation of ECM.ECM plays very important role in cell physiological function.It is not only the necessary substances using for cell adhering and supporting,but also directly affects nutrition,metabolize, proliferation,differentiation and transference.Through ECM,a lot of cytokines play the function of adjustment and control onto growth,proliferation and differentiation. Now,TGF-β(transforming growth factorβ) is recognized the key media of glomerular sclerosis.According to the traditional Chinese medicine theory,we use CTBP(Compound Tongluo Baoshen Prescription) to do the experiment to prevent and cure glomerular sclerosis.The CTBP is composed of 8 kinds of Chinese herbal:Heterophylly Falsesatarwort Root,Glossy Privet Fruit,Salvia miltiorrhiza,Rhubarb,Leech, Twotoothed Achyranthes Root and Notoginseng etc.It can invigorate the kidney and drive away evil,promote blood circulation to remove meridian obstruction.This is a start to the series study and its aim is to discuss the process and mechanism of this prescription in prevention and cure of glomerular sclerosis.1 ObjectiveIn order to offer accurate and scientific experimental evidences to the clinic prevention and cure of glomerular sclerosis,this experiment carries through the study by taking drug serum as medicine added LPS as stimulus by blood serum pathological method improved,then investigates further the effect of Compound Tongluo Baoshen Prescription on expression of TGF-β,FN and LN secreted by cultured rats glomerular mesangial cells.2 Methods2.1 To select GMCs(glomerular mesangial cells) and after it is stimulated by LPS,the rats were given intervention of CTBP with drug serum.Check GMCs proliferation by MTT methods at 12h,24h,48h and 60h.2.2 To select GMCs and after it is stimulated by LPS,the rats were given intervention of CTBP with drug serum.The TGF protein was detected in rats renal tissue by immune histochemistry staining and western blot.Detect LN and FN expressed in GMCs by Elisa experiment method.3 Results3.1 LPS can evidently promote the proliferation of GMCs.CTBP drug serum can restrain the proliferation of GMCs stimulated by LPS.3.2 CTBP can prominently inhibit the abnormal expression of TGF-β.3.3 CTBP drug serum can prominently inhibit the main content of FN and LN of ECM that secreted from GMCs stimulated by LPS.4 Conclusions4.1 CTBP can inhibit the proliferation of GMCs.4.2 CTBP can inhibit the expression of TGF-βstimulated by LPS and mitigate the accumulation of ECM.4.3 CTBP can modulate the expression of FN and LN in renal organ and reduce the abnormal accumulation of ECM.They are probably three of important function mechanisms of which CTBP postpones and slowdowns glomerular sclerosis.