Invigorating Kidney On Cultured Under High Glucose Conditions, Retinal M¨¹ller Cell Glutamate Uptake

Objective : To study the Glutamate up-take activity of Müller c ell and the LDHquantity released from Müller cell in normal and high glucose; to investigate the effect of The serum with BSHX decoction to the Müller cell in normal and high glucoseMethods: 1. Modified enzyme-digesting method for culturing Müller cell of Newborn-rat 2.serum with BSHX decoction was gained by the serum pharmacology method of traditional Chinese medicine .3 To detected the optical density of cultured Müller cell of Newborn-rat in the serum of BSHX decoction at different concentration in high glucose with MTT, to determine the effective concentration of the BSHX decoction - serum 4. To detected the the Lactate dehydrogenase (LDH) quantity released intothe incubation medium from cultured Müller cell with the Spectrophotometer.5. Glutamate up-take activity of Müller cell was determined , using isotopic techniques , by intracellular [~3H]labeled D, L-glutamate concentration.Results: 1 .Glutamate up-take activity of Müller cell increases can be causedrapid ly by high glucose , however this effect is temporary , a sharp decreases hapen to it with the time passing 2. the BSHX decoction - serum was discovered to be able to enhance the Glutamate up-take activity of Müller cell in normal glucose , meanwhile this beneficial effect afforded by the BSHX decoction - serum were found to be the strongest after 24 hours. 3 . the BSHX decoction - serum was discovered to be able to enhance the Glutamate up-take activity of Müller cell in high glucose , meanwhile this beneficial effect afforded by the BSHX decoction - serum were found to be the strongest after 48 hoursConclusion: 1. enhancement of glycolysis in Müller produced by high glucose In shorttime may be one of the mechanism of increases of Glutamate up-take activity of MüHer cell in high glucose 2. retinal Müller cells in culture maybe are resistant to high glucose, however persistent high glucose can result in injury of retinal Müller cells.3. the BSHX decoction - serum was discovered to be able to inhibition the down regulation of Glutamate up-take activity of Müller cell ,whitch maybe one of the mechanisms that BSHX method can prevent and cure DRP...