Hydroxy Yellow A To Promote The Abnormal Proliferation Of Human Umbilical Vein Endothelial Cell Apoptosis Mechanism

The malignant tumor severely impair people's health,according to the WHO statistics,the average dies every year in the world more than 50 hundred million populations in the malignant tumor reaches 6,900,000 people,sends the case is 8,700,000 examples newly,and the number is also increasing year by year.Pursues the safe effective anti-tumor medicine is an important topic.At present the clinical use's most anti-tumor medicine is or suppresses the tumour cell through the direct kill to serve the treatment purpose,these medicine side effect is very big.The anti-blood vessel production treatment or said that the tumor blood vessel treatment,is with the traditional tumor chemotherapy completely different treatment,its medicine function's object is not the tumour cell itself,but through suppresses or the destruction tumor blood vessel production control tumor grows, achieves the anti-tumor treatment the goal,but internationally develops the tumor blood vessel suppresses the medicine many are chemistry complex,the expense is expensive,and the poisonous side effect is strong.The research discovered that the low in price,the poisonous side effect small tradition traditional Chinese medicine has the noticeable function in the tumor treatment,withdraws the tumor blood vessel production from the traditional Chinese medicine to block the medicine,suppresses the tumor blood vessel's production,has become one of current anti-tumor traditional Chinese medicine research hot spots.The safflower blood circulation passes passes through,disperses the stasis to stop pain,on clinical uses in treating the esophageal cancer,the stomach cancer, liver cancer,cervical cancer and so on many kinds of malignant tumors,obtains the good curative effect;Hydroxyl safflower yellow pigment A(Hydroxy safflor yellow A,HSYA) is the effective component which withdraws from the safflower, but its function mechanism rarely seen related report.This research is for the purpose of discussing it to the person umbilical vein endothelial cell which exceptionally multiplies(Human umbilical veins endothelial cell,HUVEC) the influence,the research blood circulation removing extravasted blood traditional Chinese medicine suppresses the function target spot which the tumor blood vessel produces,perishes from the cell the angle to discuss HSYA weakly to the HUVEC action mechanism which exceptionally multiplies,provides the new way and the experiment for the anti-tumor blood vessel production medicine's research and development rests on.The present paper discusses HSYA from three aspects to the HUVEC influence which and the action mechanism exceptionally multiplies.(1) establishes under the stomach cancer cell line supernate stimulation the unusual multiplication blood vessel endothelial cell model.(2) using MTT,the class type cell technique and the TUNEL examination law observes HSYA to exceptionally to multiply the influence which the endothelial cell perishes weakly.(3) using Real-Time PCR, Western blot,the ELISA technology discusses HSYA to exceptionally to multiply the function link which and the mechanism the endothelial cell perishes weakly.First separates and raises HUVEC,will collect the BGC-823 stomach cancer cell line supernate in this foundation,joins the normal raise in HUVEC,the establishment exceptionally multiplies the tumor blood vessel endothelial cell model.The experiment is divided 3 groups:(1) normal group:Normal isolated culture HUVEC;(2) model group:Unusual multiplication blood vessel endothelial cell;(3) treats the group:The unusual multiplication blood vessel endothelial cell joins HSYA.The experimental results are as follows:(1) MTT test results show that the supernatant of tumor vascular endothelial cell proliferation was significantly higher than that of normal cultured endothelial cells(P＜0.01);and by adding the cell proliferation rate HSYA lower than model group(P＜0.05).(2) flow cytometry and TUNEL detection results show that:the model group and treatment group S phase cells increased significantly in each group G0-G1,G2-M,G2/G1 Not much will change.Apoptosis in the treatment group was significantly higher than the model group(P＜0.05).(3) treatment group compared with the model group Bax protein expression is higher than the model group(P＜0.05),Bcl-2 protein expression is lower than the model group(P＜0.05).Caspase-3 gene mRNA expression is higher than the model group(P＜0.05),mutant-type P53 gene mRNA expression is lower than the model group(P＜0.05).This experimental study prompt:HSYA possibly through affects the tumor blood vessel endothelial cell's vegetative cycle,the suggestion endothelial cell perishes weakly,suppresses the tumor newborn vascularization,thus displays suppresses the function which the tumor grows.This kind presses to perish the function to be possible weakly and HSYA activates the HUVEC line plastochondria which exceptionally multiplies to perish weakly the way,with adjustment mutant P53,the Caspase-3 gene and Bcl-2,Bax protein's expression concerns.The angle research and development new anti-tumor medicine which as well as to the tumor clinical care to the doctor practicing western medicine unify provides the new mentality and the experiment rests on.