The New Vanadium Compounds V-sih And V-the Nih To Reduce Type 1 Diabetic Rats And Its Cytotoxicity

Following the global economic development,structural changes of foods and drink have been altered in the different populations.Diabetes mellitus is increasing and seriously affect human health.Diabetes develops as a chronic disease due to hypoinsulinism(type 1) or insulin resistance(type 2).At present,the major clinic treatments for diabetes are insulin injection,organ transplantation and hypoglycemic drugs.However,disadvantages in all these treatments have observed.It is inconvenient for insulin injection.Organ transplantation is very expensive and prones to rejection reaction.Medication causes diarrhea,dizziness,liver failure and other side effects.Therefore,it is necessary to develop oral anti-diabetic drugs with high efficiency and low toxicity for treatment of diabetes mellitus.Objective:In this study,we evaluate biological and toxicological effects of V-SIH and V-NIH compounds on blood glucose in diabetes rats and cultures cells. The aim of the study was to investigate the glucose-lowering actioins of vanadium compouns and cytotoxicity to cultured cells.Methods:We established streptozotocin (STZ)-induced diabetic experimental animal model.Streptozotocin was intravenously administrated to male Wistar rats through tail vein injection.Then the STZ-induced diabetes rats were treated intragastrically with V-SIH or V-NIH compounds. Inorganic vanadium compounds NaVO₃ has been used as a positive control.During the whole experimental period,fluid intake,food consumption,body weight and blood glucose level of experimental animals were monitored in a fixed interval.At the end of experiment,rats were sacrificed.The biochemical parameters in serum were determined.On the other hand,cytotoxity of V-SIH or V-NIH compounds on rat L6 myoblast cells and human hepatoma SMMC-7721 cells were inverstigated.The cell viability was examined by methyl thiazolyt tetrazolium(MTT) assay.Results:The blood glucose levels in STZ-induced diabetic rats were decreased by V-SIH treatment. Moreover,the lipid metabolism disorder and hepatic and renal disfunctions were efficiently improved by V-SIH treatment.However,no biological effects of V-NIH on diabetes were observed.In the cell expriments,we found that vanadium compounds resulted in the cellular growth in a dose-dependent manner.Vanadium compounds stimulated the cellular proliferation at low concentrations(＜1μM) and inhibited cellular growth at high concentrations(＞10μM).In addition,cytotoxity of V-SIH is higher than that of V-NIH.Conclusion:V-SIH compound can effectively reduced the blood glucose level in STZ-induced diabetic rats.The results suggest that V-SIH can be used as a potent anti-diabetic agent.In addition,organic ligands play important role in the insulin-mimetic effect of vanadium compounds.This study will be helpful to develope a new type of anti-diabetic vanadium compound.