The Hippocampus Of Bdnf, Kalirin And Nos And Chronic Stress-induced Depression Of The Relationship

Depression is a unpleasant mind and associated with significant functional disability .It is the most common psychiatry disorders especially with low sprits. Stress is an unspecific adaptive response when animals are confronted with various stimulus. Social stress such as the quickened social rhythm,increasingly furied work competition,make people hace more and more mental burdens. However, the exact mechanism underlying depression development is still elusive. Ample evidence reveals a link of stress, particularly chronic stress, with depression development. It is unknown, however, how chronic stress precipitates or induces depression. The hippocampus has recently attracted tremendous attention for the study of depression, probably because chronic stress, produces significant effects on hippocampal neurochemistry, morphology and function,Since accumulated evidence indicates a role of hippocampal dendrite atrophy in depression development, we were interested in exploring the possible contributions of intra-cellular proteins that play a critical role in dendrite formation to depression development. We selected male SD rats, observed a variety of behavioral alterations of the eight groups by a reduction in sucrose preference, body weight, locomotor activity, rearing and grooming in open field test, and increased duration of immobility in forced swimming test and we observed the expression of BDNF Kalrin iNOS and nNOS in rat hippocampal by immunohistochemistry. The resuts are as follows:1. Compare with the Control group, the behavioural ability of the CUMS rats significantly depressed, the expression of Kaliirn (n=8,P<0.01) and BDNF decrease (n=8,P<0.01) , but the expression of iNOS and nNOS increased (n=8,P<0.01) ;2. Compare with the Control group,the behavioural ability of the intra-hippocampal injection of anti-kalirin rats significantly depressed and the expression of iNOS increased (n=7,P<0.01) ;3. Compare with the Control group,the behavioural ability of the intra-hippocampal injection of BDNF inhibitor rats significantly depressed and the expression of nNOS increased (n=7,P<0.01) ;4. we gave intra-hippocampal injections of an nNOS inhibitor, which suppressed depression-like behavioral changes induced by CUMS or intra-hippocampal injection of BDNF inhibitor; we gave intra-hippocampal injections of an iNOS inhibitor, which suppressed depression-like behavioral changes induced by CUMS or intra-hippocampal injection of anti-kalirin.From the above results ,We conclude that:The causation of CUMS can lead to depression is stress can induced the expression of BDNF and Kalirin decrease and the expression of nNOS and iNOS increase. Kalirin and BDNF not only has neroprotective function of hippocampal neurons but also has a antidepressant function by inhibiting the expression of NOS during chronic stress-induced depression; CUMS induced the expression of BDNF and Kalirin decrease and the expression of nNOS and iNOS increase ,leading to NO increase in hippocampal,which may lead to depression.Those tell us that the neroprotective factors as BDNF and Kalrin protecting brain tissue by inhibiting the expression of nNOS and iNOS will be an important way to cure depression...