Establishment Of Rat Model Of Elderly Liver Depression And Insomnia, And Shu Yuan Shen Fang Role

Objective:To establish and evaluate the symptomatic model of aging liver-qi stagnation rats suffered from insomnia;To discuss the effective mechanism of SYASF in treating aging insomnia with liver-qi stagnation.Methods:The aging model induced by D-galactose injected intraperitoneally, were observed with the following indices:the change in morphology and weight,the activity of the SOD and MDA in brain tissue.The rat model with stagnation of the liver-qi was established by clamping the tail and studied by morphological variation and the content of 6-keto-PGF_1a,TXB₂ in rats blood. Suffered from insomnia with modified multiple platform method (MMPM),the model rats were evaluated by morphological change and digital video recorder.Compared to diazepam group,the hypnosis effect of SYASF was studied by the experiment of prolonging the sleeping time in mice dealed with Pentobarbital Sodium and the hypnosis experiment in mice injected with drug dose bellowing the limen.The test of spatial exploration and orientational navigation in Morris water maze was used to explore the action of SYASF on the ability of spatial learning and memory in the symptomatic model of aging liver-qi stagnation rats.To observe the effects of SYASF on the contents of Glu and GABA in the brain tissue of the symptomatic model,the levels of Glu and GABA in cerebral cortex and hypothalamus of rat brain were assayed by HPLC method in comparison to the control group and the model group and the diazepam group.Results:The old age rats in the aging group and the model group showed that the weight increased more slowly(P＜0.01),the content of the SOD and MDA in brain tissue changed greatly(P＜0.01),when compared with the control group,but no significant difference was found between the aging group and the model group.Compared with the control group,the TXB₂ and 6-keto-PGF_1acontents in the blood of Liver-qi Stagnation rats and model group rats had significant change(P＜0.01),but there were no remarkable statistics change between these two groups.By sleep deprivation, the rats had a considerable reduction of the total sleep time in the model group and the insomnia group,but the control group had normal sleep.In the hypnosis experiment,the SYASF group prolonged the sleeping time of mice cooperated with Pentobarbital Sodium(P＜0.05)and improved the asleep ratio of mice treated with drug dose bellowing the limen(P＜0.01).The diazepam group showed a significantly effect(P＜0.01)when compared with the SYASF group.In the test of spatial exploration and orientational navigation in Morris water maze,the result of the control group and the SYASF group was better than the model group and the diazepam group.It was observed with the following behaviours: the escape latency of the model group and the diazepam group on the third day and the fourth day significantly longer(P＜0.01) than the control group and the SYASF group.Compared to the model group and the diazepam group,the percentage distribution of swimming pathway closeing to the middle ring zone and center zone was markedly increased(P＜0.01)in the control group and the SYASF group.In comparison to the control group and the diazepam group,the content of Glu in the cerebral cortex and hypothalamus of rat brain increased obviously(P＜0.01)in the model group and the SYASF group,and no significant difference was found between these two groups.The level of GABA in the diazepam group and the model group had significant change(P＜0.01),but the SYASF group showed a increasing tendency(P＜0.05)when compared with the control group.The observation of the group treated with SYASF were significantly decreased(P＜0.01)in the hypothalamus and reduced(P＜0.05)in the cerebral cortex when compared with the diazepam group.Conclusion:The symptomatic model of aging liver-qi stagnation rats suffered from insomnia was established successfully.The SYASF had a preferable effect of hypnosis and could protect the ability of spatial learning and memory in the symptomatic model of aging liver-qi stagnation rats at a certain extent. Its possible neural mechanism in treating aging insomnia with liver-qi stagnation maybe relevant to the adjusting of the activity on GABA and Glu.