TFR Of Rhepo On Ho-8910 In Ovarian Cancer Cell Surface Expression And Cell Proliferation Of

PartⅠThe Effects of rhEPO on the expression of TfR residing in the surface of Human Cancer Cell Line HO-8910Objective: To detect the expression of TfR residing in the surface of the HO-8910 cultured with rhEPO and cultured without rhEPO,accordingly to observe the effects of rhEPO on the Proliferation of Human Cancer Cell Line HO-8910.Methods: Human Cancer Cell Line HO-8910 was cultivated in RMPI-1640 medium with 10% FCS after resuscitation. After lasting passage 2d, trabant choiced six bottle of HO-8910 as experimental group and discern adding rhEPO which their saturation were discernly 50,100,200U/mL,and at the same time choiced two bottle of HO-8910 as control group without rhEPO. After 48h and 72h, collected the cell HO-8910 of each bottle and detected the expression of TfR by RT-PCR.Results:1) The main effect of two factors(rhEPO and time) have statistical significance(P<0.01). Them have interaction(P<0.01).2) The expression of TfR of HO-8910 of each bottle at the different time: When treated for 48h, experimental groupⅠ(50 U/mL): (0.878±0.016,P<0.01); experimental groupⅡ(100 U/mL): (0.954±0.047,P<0.01), which was decreased significantly; experimental groupⅢ(200 U/mL): (1.029±0.041 , P<0.01), which was decreased. When treated for 72h, experimental groupⅠ( 1.443±0.047, P<0.01); experimental groupⅡ: (1.446±0.034,P<0.01), which was also decreased significantly; experimental groupⅢ: (1.430±0.016,P<0.01).Among different time points, the expression of TfR gradually increased from 24h to 48h.Conclusion: The surface of Human Cancer Cell Line HO-8910 has expressed TfR ,and treated by rhEPO which their saturation were high, the expression of TfR residing in the surface of HO-8910 was also decreased significantly ,the conclusion hint that rhEPO is not enhance the growth of HO-8910, but also depress indirectly the growth of HO-8910 by decreasing the expression of TfR residing in the surface of HO-8910. PartⅡThe Effects of rhEPO on the cell cycle of Human Cancer Cell Line HO-8910Objective: To detect the cell cycle of the HO-8910 cultured with rhEPO and cultured without rhEPO, to approach the effects of rhEPO on the Proliferation of Human Cancer Cell Line HO-8910 and the therapeutic value on anaemia.Methods: Human Cancer Cell Line HO-8910 was cultivated in RMPI-1640 medium with 10% FCS after resuscitation. After lasting passage 2d, trabant choiced six bottle of HO-8910 as experimental group and discern adding rhEPO which their saturation were discernly 50,100,200U/mL,and at the same time choiced two bottle of HO-8910 as control group without rhEPO. After 48h and 72h, collected the cells from bottles and detected the cell cycle of the HO-8910 by FCM.Results:1) The main effect of two factors(rhEPO and time) have statistical significance(P<0.01). Them have interaction(P<0.01).2) The percentage of the S cell cycle of HO-8910 of each bottle at the different time: When treated for 48h, experimental groupⅠ( 50 U/mL): (28.21±0.09 , P<0.01); experimental groupⅡ( 100 U/mL): (28.19±0.08,P<0.01), which was decreased significantly; experimental groupⅢ( 200 U/mL): (28.24±0.09,P<0.01), which was decreased. When treated for 72h, experimental groupⅠ(31.58±0.09, P<0.01); experimental groupⅡ: (31.65±0.07,P<0.01), which was also decreased significantly; experimental experimental groupⅢ: (31.59±0.42,P<0.01), Among different time points, the percentage of the S cell cycle of HO-8910 gradually increased from 24h to 48h.Conclusion: After treated by rhEPO which their saturation were high, the percentage of the S cell cycle of HO-8910 of experimental group was decreased than of control group. The conclusion confirm that rhEPO is not enhance the growth of HO-8910, but also depress indirectly the growth of HO-8910.