Ginsenoside Rb1 On Rat Il-1¦Â And Tnf-¦Á Expression And Sleep Eeg Impact

Ginsenoside Rb1 (GsRb1) is a main effective component of Ginsenoside which can significantly improve immune function and has an inhibitory effect on the central nervous system (CNS). Two cytokines, interleukin-1beta ( IL-1β) and tumour necrosis factor-alpha ( TNF-α), which can improve immune function, play an important role in the regulation of sleep, mainly promoted the SWS. However, there was no systematic study of the relationship among Gs Rb1, the expression of IL-1βand TNF-αand sleep.In the present study, RT-PCR and immunohistochemistry was adapted to detect differentially-expressed genes modulated by different doses of ginsenoside Rb1 in the hippocampus and hypothalamus of rat. At the same time, we analyzed the slow wave sleep (SWS) of the electroencephalogram (ECoG), try to explore the mechanism how Gs Rb1 could improve the structure of sleep through cytokines.The results of RT-PCR showed that the dose (40 mg/kg) and high-dose (80 mg/kg) Gs Rb1 can significantly increase the IL-1βmRNA expression (P<0.01) in hippocampus, the dose (40 mg/kg) can significantly increase the IL-1βmRNA expression in hypothalamus, while high-dose (80 mg/kg) Gs Rb1 can significantly increase the TNF-αmRNA expression (P<0.01) in hypothalamus; but the results of immunohistochemistry showed that IL-1βand TNF-αexpression were significantly increased (P<0.01) by the three dose (20,40,80 mg/kg), as the dose increases. The analysis slow wave sleep and slow wave power spectrum of rat ECoG showed that different doses of Gs Rb1 to increase in slow wave sleep (SWS) of the total sleep time (TST) ratio, can increase the power of theδwave,αwave andθwave.We observed that Gs Rb1 to adjust the expression of IL-1βand TNF-αin hippocampus and hypothalamus of rat, and changes sructural of sleep and EEG power spectrum, which suggest that GsRb1 change the state of sleep may be through cytokines.