Affect The Mechanisms Of Power For Intracerebral Hemorrhage Model In The Rat Brain Complement C3, C4-mediated Inflammatory Response

Objective:Intracerebral hemorrhage is common clinical cerebrovascular disease,morbidity, mortality and disability higher,serious threat to human health.In recent years,more and more evidence indicate that the immune factors and inflammation play an important role in the path physiology of cerebral hemorrhage,but the complement system activation is one of the important factors of pathology damage mechanism in Intracerebral hemorrhage This reaserch in the current study of acupuncture treatment of stroke in the current,on the basis of integration,Western medicine on the stroke of the new understanding of the pathogenesis of,research complement C3,C4-mediated inflammatory response in the brain hemorrhage after the complement cascade and the important role of electro-acupuncture,the shuigou,neiguan,Zusanli of intracerebral hemorrhage rats complement-mediated inflammatory response and the impact of the mechanism.Methods:Choose healthy 60 Wistar rats(average weight of 200-250 g),male and female. The rats number,check random number table,divided into four groups:normal,the sham group,model group and electro-acupuncture group.According to bleeding time, the normal group,the sham group were divided into 3 h,24h,72h groups,each with two.Model group,electro-acupuncture treatment group were divided into 3 h,24h, 72h groups,each with eight.Model group used 0.4ul collagensⅦinjected rats caudate nucleus(0.2 mm behind the anterior fontanelle,2.9mm away from the midsagittal line at right,deep 6 mm)induced cerebral hemorrhage approach.Model group underwent sampling after 3 h.24h.72h in model.Electro-acupuncture group used electro-acupuncture treatment of cerebral hemorrhage in rats.from Point ditch Point.the Neiguan(bilateral),Zusanli(bilateral). EA 3h.24h.72h respectively in the model immediately after acupuncture once.and then every 12 hours of acupuncture 1.3h electro-acupuncture group.a total of acupuncture once.electro-acupuncture group a total of 24h acupuncture were three times,electro-acupuncture 72h acupuncture group were seven times.Ditch in the right ear and then a pair of electrodes,the same side in the custolns and Zusanli to a pail of electrodes,continuous wave.the frequency of 120/min,intensity of 1 mA,each stay-30 min.Then underwent sampling after 3 h.24h.72h in model.Above four groups of rats with 10 percent chloral hydrate aldehyde intraperitoneal injection of anesthesia,the thoracic aorta were injected saline 100 ml. 4℃pre-cooling of more than 4 percent of the POM 0.01 mol/L phosphate buffer (PBS,pH7.4)400ml.Inigation the brain,at 4 percent Polyformaldehyde solution after fixed overnight.Injecting coronavirus as the center point paraffin sections, thick-5μm,cut 10.By immunohistochemistry method of measuring brain tissue complement C3,C4,at 400 times the high-power microscope random from around the hematoma four non-overlapping vision,counting complement C3 and C4 complement positive cells,statistical analysis.Observe signs of nerve defect and score.1.Model and electro-acupuncture group of behavioral score in the comparison 3 h electro-acupuncture group of behavioral score than 3 h model group lower,but no significant difference(P＞0.05);24h electro-acupuncture group than the Model 24 h was significantly lower(P＜0.05),there are significant differences;72 h electro-acupuncture group than the Model 72 h was significantly lower(P＜0.05),a significant difference.2.Each group brain complement C3 expression 3 h lnodel complement C3 expression sham group had no significant change(P＞0.05).no significant difference;24 h model than the shaln group increased significantly(P＜0.05).There was a significant difference;72 h inodel than the sham group increased significantly(P＜0.01).a very significant difference;3 h electro-acupuncture group complement C3 expression than 3 h model group lower,no significant difference(P＞0.05);24h electro-acupuncture group than the lower 24 h model group(P＜0.05).there are significant differences:72 h electro-acupuncture group than the Model 72 h was significantly lower(P＜0.01).a very siganificant difference.3.Brain tissue of each group C4 complement the expression 3h model complement difference;24 h model than the sham group increased significantly(P＜0.05),There was a significant difference:72 h model than the sham group increased significantly (P＜0.01),a very significant difference;3 h electro-acupuncture group complement C4 expression 3 h model group lower,no significam difference(P＞0.05);24h electro-acupuncture group than the lower 24 h model group(P＞0.05),there are significant differences;72 h electro-acupuncture group than the Model 72 h was significantly lower(P＜0.01),a very significant difference.4.Cerebral hemorrhage after 3 h,24h,72h hematoma surrounding brain tissue complement C3,C4 expression analysis Cerebral hemorrhage after 24 h,72h hematoma surrounding brain tissue complement C3,C4 positive correlation between the expression,and cerebral hemorrhage after 3 h complement C3,C4 expression of a negative correlation.Conclusion:1.Complement system activation involved in the inflammatory response after cerebral hemorrhage,cerebral hemorrhage injury may be an important factor.2.Complement component C3.C4 cerebral hemorrhage in the brain tissue of rats in the dynamic changes in consistency.3.Electroacupuncture ditch,the customs,Zusanli can improve brain hemorrhage in the nerve defect of signs.4.24h electro-acupuncture group,72 h electro-acupuncture treatment group than 3 h electro-acupuncture group treatment,and may be 3 h at the complement system activation mechanism has not yet been activated due.and 24 h electro-acupuncture group.72 h electro-acupuncture was significantly lower cerebral hemorrhage Rats in the brain tissue complement C3.C4 in that acute cerebral hemorrhage early electro-acupuncture therapy can effectively reduce inflammation and thus reduce the reach of acute cerebral hemorrhage cerebral edema.