Electroacupuncture At Neiguan On Rat Ecg And Ventricular Tachycardia Study On Effect Of Plasma Catecholamine Content

Objective:Ventricular tachycardia(VT)is one of malignant arrhythmia with the feature of high hazardness,high mortality rate and high mutilation rate.A great deal of clinical researches and empirical studies show that acupuncture has unique curative effect on this disease.To approach its' mechanism of action,we carded out this experiment. The SD rats were objects in this experiment.We made rat VT models with cesium chloride(CsCl)injected into femoral vein.And them with electro-acupuncture(EA) on Neiguan(PC 6).We observed those VT rats' heart rate change and the lasting time for arrhythmia.And we detected the contents of norepinephrine (NE),epinephrine(E)and dopamine(DA)in the rats' blood plasma respectively.In this way,we are trying to explore the mechanism of acupuncture therapy in VT.Methods:60 SD rats(male or female,weighing 2.5-3.0 kilograms)were randomized into 4 groups as Normal Group(n=10),Sham Operation Group(n=10),Model Group (n=20)and Model EA Group(n=20).We made ventricular tachycardia models with cesium chloride(CsCl)injected into femoral vein.Model EA Group were treated by EA on bilateral Neiguan(PC 6)with condensed and rarefactive wave, 2Hz/15Hz,1mA for 5 minutes.For 10 of Model Group and 10 of Model EA Group, heart rate change and the lasting time for arrhythmia were observed.And the contents of NE,E and DA in the rats' blood plasma were were determined respectively.Results:1.VT rats' heart rate increased obviously after CsCl injected into femoral vein(P＜0.01).After VT model had been built successfully for 6 minutes,Model Group's heart rate changed little,but Model EA group's heart rate reduced greatly.There were obvious difference between the two groups in heart rate(P＜0.01).2.The duration of VT and the total duration of arhythmia with the rats in Model group were seperately 12.81±2.15 minutes and 45.57±14.19 minutes,while the Model EA were seperately 9.53±1.62 minutes and 30.75±9.73 minutes.Compared with the Model group,the duration of the latter was abbreviated obviously(P＜0.01 and P＜0.05).3.Compared with Sham operated group and normal group,the contents of NE,E and DA in blood plasma of Model Group increased obviously(P＜0.01).This implyed that the contents of NE,E and DA in blood plasma had abnormality variation after rats being VT.Compared with Model Group,the contents of NE,E in rats' blood plasma of Model EA Group decreased greatly(P＜0.01),and the content of DA decreased obviously(P＜0.05).Conclusion:1.The therapy of electro-acupunture(EA)on neiguan acupoint obviously decreased the ventricular tachycardia rats' heart rate,indicating that EA can antagonize arhythmia.2.The content of NE,E,DA rose markedly after VT,indicating that NE,E,DA took the pathologic diagnosis of VT.3.Compared with the Model group,the content of NE,E,DA in Model EA group in the rats' plasma decreased obviously,and approximately approached to the nomal level,indicating that EA on neiguan acupoint can markedly regulate the content of NE,E,DA in the rats' plasma,its mechanism maybe that EA on neiguan acupoint can inhibit CA's dilivery,decrease intracellular Ca²⁺,by affecting onto the sympathetic nervous system(SNS)so as to regulate rat's heart rate and antagonize arhythmia.4.The role of catecholamine(CA)in EA on neiguan(PC 6)antagonizing arhythmia provides theoretical basis for the relation of neiguan-heart.