| Vascular dementia(VD)is an impairment of cognitive function caused by cerebrovascular disease,which is one of the main styles of the aged dementias. Cognitive impairment,decreased the ability of memory and change of the Psychological signs are its main clinical manifestation.Although there is not VD's name in traditional Chinese medicine(YCM),VD belongs to "idiotia","foolish disease","stupid disease","mind disease","dementia","depressed syndrome" in TCM age-old record.Owing to so high incidence of VD,and it has the high incidence of Mutilation disease.the quality of life is affected seriously,which further results in great social and family burden.The modem medicine has maken great development in VD,many researches have been made on VD,but there is not effective methods and medicine for VD.To control the development of pathogenetic condition and to improve brain function and quality of life are main goals of treatment on clinically,and it will spend much expense for long term therapy.Recently,as more and more basic and clinic researches on Traditional Chinese Medicine(TCM)treating VD have been made,more and more privileges have appeared,k is significant for adopting traditional Chinese drug to treat VD.TCM has accumulated a lot of experience.Naoliqing capsule is according to that "deficiency of kidney-essence,obstruction of collaterals by blood stasis" is one of the main pathogenesis of VD and the therapeutical principle of VD should be "tonified kidney-essence,disperse blood stasis and dredge collateral" on the basis of clinical experience.Naoliqing capsule is made from Desertliving Cistanche,Asiatic Cornelian Cherry Frui,Sanchi,ginkgo leaf,Kudzuvine Root.Through animal experiment to expound the pharmacology of Naoliqing,we will reveal the mechanism how Naoliqing treat VD.Therefore,we established VD animal model to estimate the effects of cognitive dysfunction with Naoliqing treatment fromethology and histomorphology.The experiment is departed into three parts:1 This topic Chooses healthy male SD rats to established VD animal model.The rat models of vascular dementia were made by permanent bilateral occlusion of both commen carotid arteries(2-VO),the models were divided into six groups at random: sham-operated group,model group,nicergoline group and Naoliqing large, medium,small dose group.the medicine was given to the models by intragastric administration.2 Testing learning and memory by Morris water-maze when models were treated for 30 days.3 Fixed up via perfusion of 4%paraformaldehyde solution,brain samples with HE staining and immunohistorychemistry were applied to analyze the state of pyramidal cell and expression of ChAT and Syn.Take count of the cells and Observe the appearance of acethylcholine ChAT and Syn in CA1 district in hippocampus and compare it.We take the Escape latency of MWM as the evaluation target.Compared with model group,the large dose group and nicergoline group's escape incubation period is shorter(P＜0.01);compared with small group,the large group and medium group's escape incubation period were shorter(P＜0.01).The quantity of pyramidal cells in hippocampal CA1 region in model group was significantly less than other group's. The large group's and The medium group's the positive expression of ChAT and Syn in hippocampal CA1 region is significantly higer than model group and small dose group(P＜0.01).there was no significantly difference between small dose group and model group(P＞0.05).The Results of experiment shows that:2-VO could cause the impairment of learning and memory ability of model rats and brain tissue degeneration of VD model rats,so it succeeded in establishing VD animal models.Treating VD rats with Naoliqing capsule can obviously improve the function of spatial learning and memory of VD animal models,alleviating the impairment of neuron ischemia in the hippocampal CA1 region and enhance expression of ChAT and Syn in hippocampal CA1 region maybe the histopathological base for improving the cognitive dysfunction of VD rats. |
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