Light Silver Brain Capsule Quality Control And Scutellarin Pharmacokinetic Study

Dengyinnaotong capsule,consists of Herb Erigeron breviscapus,Leaves Ginkgo biloba,Radix Schisandra propinqua,and Radix Panax notoginseng,is one of traditional Chinese medicine recipe for promoting blood flow and dispelling wind-evil.In this dissertation,methods of quality control and the pharmacokinetics of puerarin in different decoction were studied.Quality control methods of Dengyinnaotong capsule were established by HPLC methods.HPLC-ELSD methods were established to determine,simultaneously, bilobalide,ginkgolide A,notoginsenoside R₁ and ginsenoside Rg₁ in Dengyinnaotong capsule.The linear ranges were 0.02 mg·mL^-1～0.2 mg·mL^-1(r=0.999 9),0.126 mg·mL^-1～1.26 mg·mL^-1(r=0.999 7),0.02 mg·mL^-1～0.2 mg·mL^-1(r=0.999 9)å'Œ0.02 mg·mL^-1～0.2 mg·mL^-1(r=0.999 9),respectively.The recoveries were 100.0 %(RSD=0.78%),99.9%(RSD=0.22%),99.8%(RSD=0.38%)and 100.1%(RSD=0.21 %),respectively.The contents were 0.303 mg.capsule^-1,2.107 mg·capsule^-1,1.618 mg.capsule^-1,5.008 mg.capsule^-1,respectively.RP-HPLC methods were established to determine scutellarin in Dengyinnaotong capsule.The linear range was 2.234～22.34μtg·mL^-1(r=0.999 9),The recovery was 101.6%(RSD=1.71%),The content was 15.27 mg·capsule^-1.HPLC-UV methods were established to determine,simultaneously, quercetin,kaempferol,isorhamnetin in Dengyinnaotong capsule.The linear ranges were 0.0151～0.151mg·mL^-1(r=0.999 7),0.00616～0.0616 mg·mL^-1(r=0.999 9)å'Œ0.00016～0.0016 mg·mLl(r = 0.999 6),respectively.The recoveries were 99.0 %(RSD=1.9%),103.7%(RSD=1.2%),104.6%(RSD=2.4%),respectively.The contents were 10.78 mg.capsule^-1,7.082 mg.capsule^-1,0.140 mg-capsule^-1, respectively.The assay methods are simple,rapid and with good reproducibility and provide a quantitative basis for the quality assessment for Dengyinnaotong capsule.An HPLC method was established to determine the concentration of scutellarin in rat plama.The plasma was precipicated after acidification with 5mol/L phosphonic solution. The internal stardand was naringin and the seperation was performed on a Diamonsil C₁₈(200 mm×4.6mm,5μm,Dikma)column at 35℃.The mobile phase consisted of acetonitrile-0.1%phosphonic acid solution((20:80,v/v))at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 335 nm and the injection volume was 20μL.The liner range was 0.200～20.0μg·mL^-1(r≥0.995 3),the extration recovery was more than 76.2%.Both the intra-day precision and the inter-day precision were less than 9.14%. The validated method is qualified for the pharmacokinetic study of scutellarin in rats.The pharmacokinetics of scutellarin in rats was studied after i.g.Dengyinnaotong capsule.The main parameters were as follows:t_max8.17 h,C_max5.934μg·mL^-1,AUC_0-t 81.8μg·h·mL^-1,AUC_0-∞84.1μg·h·mL^-1,t_1/23.98 h.