Yaotongding Vitro Observation, Induced Rat Bone Marrow Mesenchymal Stem Cells Into Nerve Cells

ObjectiveThis experiment approach rat-MSCs which are fractional cultivation,generationin vitro, and in Chinese crude drug circumstance to derivation rat-MSCs nerve celldifferentiated, to explore the front view of architecture engineering recovery byChinese crude drug.MethodsTakes the big rat marrow mesenchyma stem cell from the SD big rat to carryon in original generation of cultivation, divides the traditional Chinese medicineexperimental group, the medicine experimental group and the blank control groupcarries on the transfer of generation cultivation, in which traditional Chinesemedicine experimental group joins the traditional Chinese medicine lumbago todecide the induction to cultivate the big rat MSCs direction detection nerve celldifferentiation, the medicine experimental group joinsβME induction to raise thebig rat MSCs direction detection nerve cell differentiation, three groups separatelyobserve the neural cell shape under the microscope as well as carry on theimmune cell chemical analysis, separately to three group of experimental resultcomparative analysis, draws the conclusion.ResultsWith raises MSCs including 10% fetal bovine serum (FBS) L-DMEM, the originalgeneration of cell pastes the wall to grow, the cellular form is homogeneous, forspindle shape. The transfer of generation raise increases the generation to the 3rdgeneration when uses to include the non-blood serum DMEM culture medium andwith qiu the base ethyl alcohol induction raise separately which the lumbagodecides, has the obvious morphology change along with the time passage obviouscell, the cell body contraction, the diffraction enhancement, the cell appearsbreaks out. After 5 h the majority of cells present the polygon, the cone-shape orthe star type, is similar with the nerve cell, then the cell multiplication by slows down quickly. rMSC loses the multiplication ability after the induction, but stillmight continue in vitro to survive about 2 weeks. MSCs after the inductiondifferentiation, the immunity group analysis demonstrated NSE and nestin, splitsup into the neuron cell.ConclusionThis research has the exploratory by the traditional Chinese medicine effectivecomponent in vitro direction detection induction marrow mesenchyma stem cell tothe nerve cell differentiation, the innovation. Not only the YaoTongDing decides theeffective traditional Chinese medicine ingredient to be possible to cause the MSCsdirection detection to split up into the primitive nerve cell, moreover is helpful toneural cell in long-term survival. The YaoTongDing decides the effective traditionalChinese medicine ingredient to the MSC long-term growth in non-toxicdifferentiation, may take further the quite ideal derivative in clinical neural celltransplant.