Different Channels. Sivmac251 Infection Of Chinese Rhesus Experiments

Sexual transmission is the dominant route for epidemic spread of humanimmunodeficiency virus type 1 (HIV-1), animal model created by mucosaroute will be a need for evaluation on certain vaccines agaist HIV. In thepresent stage, there is a lack of the systemic comparative research onSIV/SAIDS rhesus monkeys infected by intravenous, intrarectal andintravagina routes respectively in china. In this research, we used SIVmac251to inoculate rhesus monkeys by intravenous, intravagina and intravectalroutes for studying the infection situation, whether if any differences of theclinical manifestation, the virology and immunology detections and the SIVgene variation occur ammong the different routes.Three rhesus monkeys were inoculated with SIVmac251 by intravenous,intravagina and intrarectal, respectively. We have confirmed that theseexperimental monkeys had been infected successfully by testing virusisolation, virus load in plasma, pro-virus DNA in PBMCs and the ratio ofCD4~+/CD8~+.The experimental monkeys inoculated by different routes had anorexiaphenomenon from 14d after infection and lasted for two weeks. There werelosing weight and lymph node enlargement. S 153 died in half year attendingby anorexia, losing weight, diarrhea and lymph node atrophy. The firstdetectable virus in three monkeys were S153(3d), S172(7d) and S142(10d),respectively. All viremia peaks appeared at 14d ater infection, then declinedrapidly. Virus isolation showed longer duration of virus peaks in mucosa route monkeys.The antibody became positive in S153 from 10th days and continuouslyremained very low level in the entire infection period, the highest titre was at1:80. The antibodies were positive in S142 and S172 from 14th days, theantibody levels of S142 and S172 were continuously very high in the entireinfection, The highest value of S 142 was 1:5120, and S 172 was 1:2560.The virus loads of S142 and S172 in the lymph tissue were very high,S142 was 1.118E+08, S172 was 1.288E+07. The pathological section oflymph node revealed sevious damages in lymph tissues, but the virus loadsfrom plasma were very low, S142 was 4.322E+03, S172 was negative.The CD4~+/CD8~+ ratio droped, and appeared the proportion inversion in the28d after the infection.The pathogenesis and SIV process in vivo still remains unknown, thepossible variation of SIV genome may occur during monkey infection,roughly, we chose nef and env vlv2 from the SIV as target gene to check ifhas any signicant changes from SIV from three infected monkeys.In conclusion, the Chinese macaque were successfully infected withSIVmac251 by different routes, and the results from clinic, diseasedevelopment, virology and immunology examinations, pathologic changesand SIV variation have shown different outcome, leading us to the furtherresearch.