Evil Theoretical Discussion Of Medicine "poison" From The "poison" On The Rule Of Non-alcoholic Fatty Liver Experimental Study

ObjectivesWith the improvement of the standard of living, more and more people are suffering nonalcoholic fatty liver disease (NAFLD).The mechanism of NAFLD is complicated and still unclear. There aren't any ideal medications to prevent NAFLD.Chinese traditional medicine(TCM) possesses some superiority on it.The earlierperiod clinical curative effect observation indicated PDJZ mixture has the obviousfunction of improvement liver function and reduces the blood fats. In this foundation, using the model of NASH recognized by the world and linking to the evolution of modern research of NASH,we observe serum alanine aminotransferase (ALT),aspartate aminotransferase (AST),low density lipoprotein (LDL),high density lipoprotein (HDL),total cholesterol(TC),triglyceride(TG),superoxide dismutase (SOD),malondialdehyde(MDA) the tumor necrosis factor-α(TNF-α) and Liver pathologicalsection of NAFLD rats to study function mechanism of PDJZ treating NASH,provideing the experiment for the clinical use.MethodsSixty clean graded male SD rats,weight 190±20g.were feed 1W normally, thenwere randomly divided into 6 groups,namely normal control group(A),blank roodel group(B),PDJZ mixture groups:low dose,common dose,high dose (C1,C2,C3) and ZHIBITUO Tablets group(D).To confect high fat diet replicate NASH model ofrats to dilute PDJZ mixture to different concentration of 1:2:4.After 8W, fed the rats of every model group with foods rich in fat unceasingly, to pour these suspension of PDJZ mixture into stomach of rats of C1,C2,C3 groups separately, 1ml/100g.d,qid;the rats of D group are poured suspension of ZHIBITUO tablet into stomach,0.09g/1ml/100g.d,qid;the rats of B group are poured isotonic nachioride intostomach, 1ml/100g.d,qid.At the end of twelve week of the experiment,all rats are f asted for 12h,then killed after being narcotized with pentobarbitale sodium to leave over serum and hepatic tissue rapidly according to routine.Then observed common state of animal when the models are being replicated,reckoned liver index,to detect the content of serum ALT, AST, TC,TG, LDL,HDL. To leave over 1g of hepatic tissue from right lobe of liver to make 10% liver homogenate at 4℃for detecting content of SOD,MDA.Hepatic tissue is fasted by formalin,packed with paraffin,sectioed,stained with HE,evaluated the degree of hepatic steatosis and inflammationunder the optical microscope.Hepatic tissue was fasted by formalin,packaged witholefin,sectioed,to detect the expression of TNF-α.Result1.According to the hepatic tissue pathology and the state of hepatic functionof rats,we succeeded in replicating the models of rats with NASH.Compared withthat of the blank model group,the liver index of high and common dose of PDJZ mixture group decrease significantly(P＜0.01) the level of serum ALT, AST, TC,TG,LDL decrease markedly(P＜0.01 or P＜0.05),elevates markedly(P＜0.01 or P＜0.05)thelevel of serum HDL,superor to that of medlar fruit, silybin and ZHIBITUO tablet group(P＜0.01 or P＜0.05).2.The effect of PDJZ mixture on the state of oxidation/anti- oxidation:Compared with that of the blank model group,the level of hepatic SOD of PDJZ mixturegroup high dose group increased significantly (P＜0.01),the content of MDA decreased significantly(P＜0.01),superor to that of ZHIBITUO tablet group(P＜0.05).3. The effect of PDJZ mixture on the hepatic tissue expression ofTNF-αprotein:Compared with that of the blank model group,the hepatic tissue expressionof TNF-αof PDJZ mixture high dose group decreased significantly(P＜0.01), superor tothat of ZHIBITUO tablet group(P＜0.01).4.Under the optical micro(?)ope,normal group big mouse liver organizational st ructure integrity, clear,the structure of liver small leaf is normal,the central vein isgreat but the wall book,the liver cell assumes the radiated distribution around thecentral vein,the cell assumes the polygon.All specimens liver tissue slice of modelgroup rats obviously fill the air liver cell fat denaturation, simultaneously liver small leaf in and collects the district have the uninuclear cell primarily inflammation germ cell to infiltrate,by small leaf in inflammation primarily.In partial rats liver organization small leaf necrosis stove fusion Large expanseof. Rats liver cell moderate cell dropsy.IN high and common dose of PDJZ mixture,structure of liversmall leaf clear, cell arrangement neat, only has in the minority liver cell to have the fatdrop vacuole,the cell nucleus structure clearly, the dyeing is normal.In the low dose of PDJZ mixture and the ZHIBUTUO tablet,liver cell shape not be irregular in the Cytoplasm.The fat-drop obviously increases,the chromosome is mild swelling.Conclusion1.The effect on the hepatic tissue pathology and the state of hepatic function:PDJZ mixture can decrease the liver index and and the level of serum ALT andAST of rats with NASH,superior to that of ZHIBITUO tablet group.It shows thatPDJZ mixture can prevent NASH.2.The effect on the content of blood fat: PDJZ mixture can decrease the content ofserum TC,TG;LDL and increase level of HDL,so that reduce the toxicity of theaccumulation of TC and TG.3. The effect on the state of oxidation/anti-oxidation:PDJZ mixture can increase theability of anti-oxidation,relax oxidative stress,reduce lipid peroxidation.4.The effect on the hepatic tissue expression of TNF-αprotein:PDJZ mixturePreparation can decrease the expression of TNF-αprotein in hepatic tissues,so that reduce the injure to CKs.PDJZ mixture to be able through to reduce NAFLD blood serum ALT, AST, TC,TG;LDL,increase HDL to protect the liver.The possible mechanism of protectingthe liver by enhancing NASH rats' SOD,reducing the MDA level,enhancing the 1iver to organize the anti-oxidized ability, alleviating the oxidation to be supposed tostir up,reduces the lipin peroxidation,also suppressing the NASH rats' liver to organize the TNF-αexpression to reduce the liver inflammation and the damage.