Circulation Expectorant Particles On The Protective Effect Of Acute Myocardial Ischemia And Myocardial Microcirculation

OBJECTIVE (1)To simulate two kinds of pathophysiology process whichwere coronary artery microembolization (CAM) and no reflowphenomenon in a new CAM by injecting pituitrin(PIT) (2)To prove thatmicrangium vasospasm of Cardiac muscle (CM) could cause micrangiumlumina narrow, endotheliocyte damage, thrombocyticactivation ,hemorheological extraordinary, which can producemicrothrombus and induce cardiac muscle microcirculation disorder.(3)Resolving stasis eliminating phlegm granule are proved to obviateCM microcirculation disorder by protecting blood vessel endotheliumfunction, preventing coronary artery vasospasm, depressingthrombocytic activation and hemorheological extraordinary.METHOD 40 rats were divided into 4 groups by random, 10 eachgroup. 1)blank control group: normal sodium chloride wasintragastric administrated .2) Model control group: the same togroup 1. 3)Herbesser control group: 2.7mg/kg Herbesser wereintragastric administrated. 4) Resolving stasis eliminating phlegmgranule control group: 18.9mg/kg Resolving stasis eliminatingphlegm granule were intragastric administrated. The models of CMmicrocirculation disorder were founded by injecting 1.5U/kg PIT intothe rats' sublingual vein after 4 weeks intragastric administrated.After the experiment, the changes of the rats' ECG J point in eachgroup were monitoried; the changes of rats' CM ultrastructure wereobserved; the cross section area(CSA)of microcirculation vesselwere measured; the NO and ET saturation in rats' blood plasma weredetected.RESULT Compared with blank control group, the rats' ECG J pointelevated obviously in model control group , and CM ultrastructurewere destroyed, CSA of microcirculation vessel decreasedobviously ,the NO saturation decreased but the ET saturationincreased in rats' blood plasma. Compared with model control group,the rats' ECG J point didn't elevated obviously in both Herbessercontrol group and Resolving stasis eliminating phlegm granulecontrol group, and return time shortened obviously ,CMultrastructure were not destroyed obviously, CSA ofmicrocirculation vessel increased obviously, the serum level of NOincreased but the serum level of ET-1 decreased. All the data in bothHerbesser control group and Resolving stasis eliminating phlegmgranule control group have no significant difference. CONCLUSION 1 A new kind of model of rat's coronary arterymicrothrombus was founded by injecting PIT into rats' sublingualvein. 2 the serum level of NO decreased but the serum level of ET-1increased after CM microcirculation disorder, which means the damageof micrangium endotheliocyte played a part in formingmicrocirculation disorder. 3 Resolving stasis eliminating phlegmgranule can obviate CM microcirculation disorder finally bydepressing thrombocytic activation, preventing coronary arteryvasospasm and obviating microthrombus, by means of protecting CMultrastructure, depressing ECG J point elevation, shortening returntime, increasing CSA of microcirculation vessel, increasing theserum level of NO but decreasing the serum level of ET-1.