Hepatoprotective Effect Of The Five Thistle Capsules (ab) Research And Mechanism

AB is made up of silymarin and Schisandra chinensis by the method of homogeneous design screening test. The best matching is 1:1. The protective effects of AB oral on liver injury were studied. The results showed that AB injection could degrade the contents of ALT, AST and the total bilirubin in serum, inhibit the production of liver fibrosis.The protective effects of AB on CCl₄-induced, D-GalN-induced or TAA-induced acute liver injury and alcohol-induced chronicity liver injury, CCl₄-induced liver fibrosis were studied, effects of AB on liver regeneration and bile secretion were also observed in this paper.The results showed that the activities of ALT, ASTwere significantly decreased in CCl₄-induced or D-GalN-induced acute liver injury mice pretreated with AB (200 mg·kg^-1, 100 mg·kg^-1, 50 mg·kg^-1 i.g.), at the same time, the contents of MDA were degraded in liver.Pretreatment with AB (130 mg·kg^-1, 65 mg·kg^-1, 32.5 mg·kg^-1 i.g.) could decreased the activities of ALT, AST in serum of acute liver injury rats induced by CCl₄, It could decrease the pathologic lesion of liver cell.Pretreatment with AB (130 mg·kg^-1, 65 mg·kg^-1, 32.5 mg·kg^-1 i.g.) could decreased the activities of ALT in serum of acute liver injury rats induced by TAA. It could also increase the quantity of TP and Alb in serum, the contents of MDA were degraded.Pretreatment with AB (200 mg·kg^-1, 100 mg·kg^-1, 50 mg·kg^-1 i.g.) could decreased the activities of ALT, AST, TG in serum and TG, MDA in liver of chronicity liver injury mice induced by alcohol.Pretreatment with AB (130 mg·kg^-1, 65 mg·kg^-1, 32.5 mg·kg^-1 i.g.) could inhibit the production of liver fibrosis on CCl₄-induced liver fibrosis in rats. The results indicated that the contents of the collagen in extracellular matrix were visibly decreased the amount of hydroxyproline in liver were reduced.Pretreatment with AB (200 mg·kg^-1, 100 mg·kg^-1, 50 mg·kg^-1 i.g.) could definite liver-protection and promoting bile secretion.We studied the acute toxicity of AB in mice, the LD₅₀ of AB (i.g.) and AB (i.p.) are 2230mg·kg and 1760mg·kg.The results of chronic toxicity of AB in rats suggest that, the toxicity dose is 1040mg·kg, the main target organs is liver.