Efficient And Stable Expression Of Recombinant Mouse Stem Cell Factor-1 15p-1 Cell Clones To Establish

Objective: establish 15p-1 cell clone which can stably and efficiently express recombinant mouse SCF1, it is expected that these cell clones will contribute to the survival, proliferation and differentiation of spermatogonial stem cells.Methods: Construct recombinant plasmid containing mouse SCF1 cDNA fragment, then introduce it to 15p-1 cells by using a non-liposomal lipid formulational reagent. Western blot is applied to analysis the protein expression of 15p-1 cell clones screened under the pressure of antibiotics selection.Results: Through Western blot , protein expression equivalent to the molecular weight of mouse SCF1 is detected among 11 15p-1 cell clones.Conclusion: The result of Western blot suggest that the design of the recombinant plasmid and the selection of transfection method are reasonable, and indicate that we have already establish stably and efficiently SCF1-expressing 15p-1 cell clone.