Znf207 Gene Expression Level In P19 Cells Induced To Differentiate

[Objective] To observe the process of cell aggregation anddifferentiation into cardiac myocytes after the P19cells treated with 0.9%dimethyl sulgoxide (DMSO) in vitro.[Methods] P19 cells were cultured and induced to differentiate tocardiac myocytes by 0.9% dimethyl sulfoxide (DMSO) in vitro. Cellmorphological changes were observed under the inverted microscope.[Results] On day 4 after DMSO-induced differentiation, cellaggregation formed. Spontaneous and rhythmically beating cells wereobserved on day 8, and the amount of fusiform cells were increasinggradually.[Conclusion]P19 cells exhibit the trend of differentiation towardscardiac myocytes in vitro in the presence of 0.9% DMSO. [Objective] To investigate the changes of zinc finger protein 207(ZNF207) gene mRNA during P19 cells' differentiation to cardiacmyocytes, and to explore the relationship between ZNF207 gene and thedifferentiation of cardiac myocytes.[Methods] P19 cells were cultured and induced to differentiate tocardiac myocytes in vitro. Total RNA were extracted from P19 cellsduring the process of differentiation at various points: pre-differentiation(Day 0), Day 1 to Day 17, and the levels of ZNF207 mRNA wereevaluated by RT-PCR.[Results] In P19 cells, the expression of ZNF207 mRNA was thelowest. When P19 cells were induced by 0.9%DMSO to differentiate tocardiac myocytes, the level of ZNF207 gene mRNA was up-regulatedand reached the highest level in day 10. There was a significant differencebetween day 2 to day 10(P＜0.05).[Conclusions] The expression of ZNF207 gene is up-regulatedduring P19 cells' differentiation to cardiac myocytes, which maycontribute to the differentiation of cardiac myocytes.