Study, The Ssangyong Particles Pharmaceutical Research And Anti-asthmatic Effect

Asthma is a common respiratory disease regarded as a one of the four chronic diseases. Compound Shuanglong(SL)granule is made of Ephedra,radix bupleuri,Chinese Magnoliavine Fruit and Earth worm,Rhizoma dioscoreae nippponicae ect.The prescription of SL granule was based upon an effective medical prescription to treat asthma.The studies include literature review,pharmaceutical research,pharmacology research.With the guide of Chinese medicine theory,conduct the analyses on the medicine of the prescription,establish the distill method and conduct procedure pick up depending on the component of Chinese medicine.1 Literature ReviewWe had reviewed asthma-related literature in home, including the following aspects: mechanism development of anti-asthma TCM , clinic research of anti-asthma TCM.2 pharmaceutical research2.1Reseracher on preparation procedureEphedra contents ephedrine chloride and Semen armeniacae amarum contents amygdalin and so on. These composition are easy to solved in water. Establish the best distill procedure with the dried extract,the content of ephedrine chloride and the content of amygdalin as the component index,select the solvent dosage,distill time,distill step and soak time,make sure to withdraw the means best.Rhizoma dioscoreae nippponicae contents dioscin and radux scytekkaruae contents baicalin and so on.These composition are easy to solved in alcohol. Establish the best distill procedure with the dried extract,the content of dioscin,baicalin as the component index,select the alcohol density,solvent dosage,distill time and distill step,make sure to withdraw the means best. Research on the shaping procedure:Review the obtain rate of grain for index. Consider the soft material kind,the granule of system is difficult easy,grain color and agglomeration synthetically,investigate the powder of medicine and the comparison of the dextrine,the smooth wet certain grain in a density of ether models the term.2.2Quantity standard researchThe characteristic compositions were taken as the the indexs with TCL to identify the quality of fructus schisandrae chinensis,Bulbus fritillariae cirrhosae,Rhizoma dioscoreae nippponicae.The content of ephedrine chloride in the granule was determined with HPLC.3 pharmacology research3.1Effect of SL granule on airwayhyper-responsiveness in asthmatic animalRats was sensitized respectively by ovalbumin (OVA) , before antigen challenge,rats or guinea pigs in treatment groups were fed with SL granules. To determine airway hyper-responsiveness Lung-function examinations were performed in asthmatic rats. In asthmatic rats,airway hyper-responsiveness was seen with a significant decrease in mean values of Fev0.2,PEF and Fev0.2/FVC compared with the control group and capsule increased these parameters obviously.These showed SL granule had the DXM-like effect on changes of lung function and improves some symptom of asthma.3.2Effect of SL granule on inflammatory cells infiltrationTo set up asthmatic model,rats was treated with a same method above. Bronchoalveolar lavage fluid (BALF) was prepared for differential white cell counts. Asthmatic rats markedly increased the percentage of eosinophils in BALF,the number in animals with drugs is lower.Data indicated that SL granule could inhibit eosinophils.In conclusion,we have established the optimum conditions of extraction process and a sensitive,accurate,repeatable and specific quality specification of SL granule .At the same time, pharmacology researches about SL granule on rats showed a result which estimated its therapy effect.