Lung Cancer Tissue Of Mir-125b-1 Gene Mutation In A Preliminary Analysis

Objective This report aimed at the mutation of miR-125b-l gene in human lung cancer,and the evaluation of its possible effect and mechanism on the development of lung cancer.Methods PCR-SSCP was used to detect miR-125b-1 gene mutation in 121 specimens from primary site of lung cancer tissues and 22 samples of normal adjacent lung tissues.Results Altogether there were 40 cases of miR-125b-1 gene mutation , and the analysis revealed that its mutation was positively correlated to the status of lymph node metastasis (rs = 0.285 , P<0.001) and clinical stage metastasis(rs = 0.241 ,P < 0.001), but no relationship was found between miR-125b-1 mutation and other clinical features.Conclusion There is miR-125b-1 gene mutation in lung cancer. miR-125b-1 gene mutation probably plays a crucial role in lung carcinogenesis.