Augmenter Of Liver Regeneration On Effect Of Human Renal Tubular Epithelial Cell Transdifferentiation

Objective: To express rALR in GS115 with the constructed pichia pastoris expression plasmid of rat augmenter of liver regeneration (rALR) and purify it with ultrafiltration and identify its bioactivity in vitro, for studying its biological functions.Methods: The recombinant plasmid pPIC9K-rALR was transformed into GS115 by electroporation. The rALR was expressed in GS115 under the induction of methanol and purified with ultrafiltration.. The purified product was identified by 15% SDS-PAGE and Western blot. The effects of rALR on the proliferations of QGY were evaluated by 3H-TdR methods in vitro.Results: The rALR was efficiently expressed as a secretive protein in GS115. Its molecular weight, with 1.5×10~4 dalton, was in correspondance with theoretic values. Single band of rALR was observed in SDS-PAGE electrophoresis and in western blot after ultrafiltration. The rALR promoted the proliferation of QGY by a dose-dependent way in vitro.