According Miding, Inhibition Of Nmd Mechanism Of The Effect And Mechanism Of P53 Gene Expression In Human Breast Cancer Cell Lines

EFFECT OF EMETINE ON THE EXPRESSION OF P53 IN HUMAN BREAST CANCER CELLSObjective: To determine if the expression of P53 gene is influenced by Emetine that inhibited the NMD mechanism in human breast cancer cells. Methods: The RT-PCR technique was applied for the assay of the expression of wild type and mutant P53 gene in MDA-MB-231 cells and MCF-7 cells.Results: The expression of mutant P53 gene was detected and increased by Emetine treatment (P<0.05).The expression of wild type P53 gene was not found in control and treat groups of MDA-MB-231 cells. We detected the wild type P53 was expressed in MCF-7 cells, and don't have great change after Emetine treatment (P>0.05). The mutant P53 were not found in control and treat groups of MCF-7 cells.Conclusion: The expression of mutant P53 was increased by Emetine treatment as compared with control groups in MDA-MB-231 cells, while the expression of wild type P53 was not great difference by Emetine treatment as compared with control groups in MCF-7 cells. The influences of Emetine on gene expression of mutant P53 than wild type P53 are relative selectivity.